OST48 Antibody (E-9): sc-74408

OST48 Antibody (E-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects OST48 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-OST48 antibody (E-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. OST48 plays a crucial role in the mammalian oligosaccharyltransferase (OST) complex, which is essential for the cotranslational N-glycosylation of newly synthesized polypeptides. This process is vital for proper protein folding, stability, and function, as glycosylation can influence protein interactions, localization, and activity. The OST complex, which includes OST48 along with ribophorins I and II and DAD1, is localized to the rough endoplasmic reticulum, where OST48 facilitates the addition of oligosaccharides to nascent proteins. The interaction between the cytoplasmic N-terminal region of DAD1 and the short cytoplasmic tail of OST48 is critical for the assembly and function of the OST complex, underscoring OST48′s importance in maintaining cellular homeostasis and protein quality control.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

OST48 Antibody (E-9) References:

1. Retention of subunits of the oligosaccharyltransferase complex in the endoplasmic reticulum.  |  Fu, J. and Kreibich, G. 2000. J Biol Chem. 275: 3984-90. PMID: 10660554
2. Localization of ribophorin II to the endoplasmic reticulum involves both its transmembrane and cytoplasmic domains.  |  Fu, J., et al. 2000. Eur J Cell Biol. 79: 219-28. PMID: 10826490
3. Lysine can be replaced by histidine but not by arginine as the ER retrieval motif for type I membrane proteins.  |  Hardt, B. and Bause, E. 2002. Biochem Biophys Res Commun. 291: 751-7. PMID: 11866428
4. The 48-kDa subunit of the mammalian oligosaccharyltransferase complex is homologous to the essential yeast protein WBP1.  |  Silberstein, S., et al. 1992. J Biol Chem. 267: 23658-63. PMID: 1429707
5. Loss of specific chaperones involved in membrane glycoprotein biosynthesis during the maturation of human erythroid progenitor cells.  |  Patterson, ST., et al. 2009. J Biol Chem. 284: 14547-57. PMID: 19258325
6. Selenoprotein K binds multiprotein complexes and is involved in the regulation of endoplasmic reticulum homeostasis.  |  Shchedrina, VA., et al. 2011. J Biol Chem. 286: 42937-48. PMID: 22016385
7. Keratinocyte-associated protein 2 is a bona fide subunit of the mammalian oligosaccharyltransferase.  |  Roboti, P. and High, S. 2012. J Cell Sci. 125: 220-32. PMID: 22266900
8. The AGE receptor, OST48 drives podocyte foot process effacement and basement membrane expansion (alters structural composition).  |  Zhuang, A., et al. 2021. Endocrinol Diabetes Metab. 4: e00278. PMID: 34277994
9. Age-dependent effects of a high-fat diet combined with dietary advanced glycation end products on cognitive function and protection with voluntary exercise.  |  Luo, L., et al. 2022. Food Funct. 13: 4445-4458. PMID: 35342920
10. DAD1, the defender against apoptotic cell death, is a subunit of the mammalian oligosaccharyltransferase.  |  Kelleher, DJ. and Gilmore, R. 1997. Proc Natl Acad Sci U S A. 94: 4994-9. PMID: 9144178
11. Interactions among subunits of the oligosaccharyltransferase complex.  |  Fu, J., et al. 1997. J Biol Chem. 272: 29687-92. PMID: 9368036
12. DAD1 is required for the function and the structural integrity of the oligosaccharyltransferase complex.  |  Sanjay, A., et al. 1998. J Biol Chem. 273: 26094-9. PMID: 9748289

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-OST48 Antibody (E-9) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-OST48 Antibody (E-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

OST48 Antibody (E-9) Specifications

Alternate Product Name: OST48

Product Clone Name: E-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 157-456

Epitope Target: OST48

Epitope Species: human

Product Reactivity/Detection: OST48

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for OST48 Antibody (E-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin