OSCP Antibody (A-8): sc-365162

OSCP Antibody (A-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects OSCP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). OSCP (A-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The oligomycin-sensitivity conferring protein (OSCP), also known as ATP5O, is a crucial component of ATP synthase, which is located in the mitochondria and plays a vital role in ATP synthesis. OSCP is essential for cellular energy production, as mitochondrial ATP synthases convert the energy from membrane electrochemical proton gradients into the high-energy phosphate bonds necessary for ATP generation. The structure of ATP synthase is composed of two linked complexes: F1, which serves as the hydrophilic catalytic core, and F0, which forms the membrane-embedded protein channel. The F1 complex consists of three alpha chains and three beta chains, along with one gamma chain, one delta chain, and one epsilon chain, while the F0 complex is made up of three subunits: a, b, and c. The epsilon chain of F1, comprising just 50 amino acids, is the smallest of the five chains in the ATPase F1 complex, highlighting the intricate design and functionality of OSCP in energy metabolism.

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OSCP Antibody (A-8) References:

1. Purification and identification of an estrogen binding protein from rat brain: oligomycin sensitivity-conferring protein (OSCP), a subunit of mitochondrial F0F1-ATP synthase/ATPase.  |  Zheng, J. and Ramirez, VD. 1999. J Steroid Biochem Mol Biol. 68: 65-75. PMID: 10215039
2. Oligomycin sensitivity-conferring protein (OSCP) of mitochondrial ATP synthase. The carboxyl-terminal region of OSCP is essential for the reconstitution of oligomycin-sensitive H(+)-ATPase.  |  Joshi, S., et al. 1992. J Biol Chem. 267: 12860-7. PMID: 1535627
3. Interactions between the oligomycin sensitivity conferring protein (OSCP) and beef heart mitochondrial F1-ATPase. 1. Study of the binding parameters with a chemically radiolabeled OSCP.  |  Dupuis, A., et al. 1985. Biochemistry. 24: 728-33. PMID: 2859882
4. Lack of ability of trypsin-treated mitochondrial F1-ATPase to bind the oligomycin-sensitivity conferring protein (OSCP).  |  Hundal, T., et al. 1983. FEBS Lett. 162: 5-10. PMID: 6225683
5. The oligomycin sensitivity conferring protein (OSCP) of beef heart mitochondria: studies of its binding to F1 and its function.  |  Hundal, T., et al. 1984. J Bioenerg Biomembr. 16: 535-50. PMID: 6242246
6. ATP synthase complex. Proximities of subunits in bovine submitochondrial particles.  |  Belogrudov, GI., et al. 1995. J Biol Chem. 270: 2053-60. PMID: 7836433
7. Membrane topography and near-neighbor relationships of the mitochondrial ATP synthase subunits e, f, and g.  |  Belogrudov, GI., et al. 1996. J Biol Chem. 271: 20340-5. PMID: 8702768
8. Oligomycin sensitivity conferring protein of mitochondrial ATP synthase: deletions in the N-terminal end cause defects in interactions with F1, while deletions in the C-terminal end cause defects in interactions with F0.  |  Joshi, S., et al. 1996. Biochemistry. 35: 12094-103. PMID: 8810915
9. Structural interactions of the oligomycin sensitivity-conferring protein in the yeast ATP synthase.  |  Mao, Y. and Mueller, DM. 1997. Arch Biochem Biophys. 337: 8-16. PMID: 8990262
10. A novel fluorescent marker for assembled mitochondria ATP synthase of yeast. OSCP subunit fused to green fluorescent protein is assembled into the complex in vivo.  |  Prescott, M., et al. 1997. FEBS Lett. 411: 97-101. PMID: 9247150
11. Oligomycin sensitivity conferring protein (OSCP) of bovine heart mitochondrial ATP synthase: high-affinity OSCP-Fo interactions require a local alpha-helix at the C-terminal end of the subunit.  |  Joshi, S., et al. 1997. Biochemistry. 36: 10936-43. PMID: 9283085
12. The oligomycin sensitivity conferring protein of rat liver mitochondrial ATP synthase: arginine 94 is important for the binding of OSCP to F1.  |  Golden, TR. and Pedersen, PL. 1998. Biochemistry. 37: 13871-81. PMID: 9753477

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-OSCP Antibody (A-8) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-OSCP Antibody (A-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

OSCP Antibody (A-8) Specifications

Alternate Product Name: OSCP

Product Clone Name: A-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 71-210

Epitope Target: OSCP

Epitope Species: human

Product Reactivity/Detection: OSCP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for OSCP Antibody (A-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin