ORP-7 Antibody (E-2): sc-515540
- ORP-7 Antibody (E-2) is a mouse monoclonal IgG1 κ ORP-7 antibody provided at 200 µg/ml
- raised against amino acids 172-310 mapping within an internal region of ORP-7 of human origin
- ORP-7 Antibody (E-2) is recommended for detection of ORP-7 of human origin and Osbpl7 of mouse and rat origin of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-ORP-7 Antibody (E-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ORP-7 Antibody (E-2) for WB applications. These reagents are now offered in bundles with ORP-7 Antibody (E-2) (see ordering information below).
ORP-7 Antibody (E-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects ORP-7 protein of human origin and Osbpl7 of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ORP-7 (E-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ORP-7, also known as OSBPL7, is a crucial member of the oxysterol-binding protein (OSBP) family, which plays a significant role in lipid metabolism and cellular signaling. ORP-7 is primarily located in the cytoplasm and translocates to the periphery of Golgi membranes upon binding to oxysterols, highlighting ORP-7′s importance in lipid transport and cellular communication. A C-terminal sterol-binding domain and an N-terminal pleckstrin homology domain in ORP-7 facilitates interaction with various lipids, including phosphatidic acid and phosphatidylinositol 3-phosphate, which are vital for maintaining cellular homeostasis and signaling pathways. Encoded by a gene on human chromosome 17, ORP-7 consists of 842 amino acids and is part of a larger family of OSBP-related proteins that are essential for regulating lipid dynamics within cells.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ORP-7 Antibody (E-2) References:
- The OSBP-related protein family in humans. | Lehto, M., et al. 2001. J Lipid Res. 42: 1203-13. PMID: 11483621
- A family of 12 human genes containing oxysterol-binding domains. | Jaworski, CJ., et al. 2001. Genomics. 78: 185-96. PMID: 11735225
- An oxysterol-binding protein family identified in the mouse. | Anniss, AM., et al. 2002. DNA Cell Biol. 21: 571-80. PMID: 12215260
- The OSBP-related proteins: a novel protein family involved in vesicle transport, cellular lipid metabolism, and cell signalling. | Lehto, M. and Olkkonen, VM. 2003. Biochim Biophys Acta. 1631: 1-11. PMID: 12573443
- Subfamily III of mammalian oxysterol-binding protein (OSBP) homologues: the expression and intracellular localization of ORP3, ORP6, and ORP7. | Lehto, M., et al. 2004. Cell Tissue Res. 315: 39-57. PMID: 14593528
- Oxysterol binding proteins: in more than one place at one time? | Olkkonen, VM. and Levine, TP. 2004. Biochem Cell Biol. 82: 87-98. PMID: 15052330
- The mammalian oxysterol-binding protein-related proteins (ORPs) bind 25-hydroxycholesterol in an evolutionarily conserved pocket. | Suchanek, M., et al. 2007. Biochem J. 405: 473-80. PMID: 17428193
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ORP-7 Antibody (E-2) | sc-515540 | 200 µg/ml | $316.00 | |||
ORP-7 Antibody (E-2): m-IgG Fc BP-HRP Bundle | sc-531404 | 200 µg Ab; 10 µg BP | $354.00 | |||
ORP-7 Antibody (E-2): m-IgGκ BP-HRP Bundle | sc-525087 | 200 µg Ab, 40 µg BP | $354.00 | |||
ORP-7 Antibody (E-2): m-IgG1 BP-HRP Bundle | sc-544532 | 200 µg Ab; 20 µg BP | $354.00 | |||
ORP-7 Antibody (E-2) AC | sc-515540 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ORP-7 Antibody (E-2) HRP | sc-515540 HRP | 200 µg/ml | $316.00 | |||
ORP-7 Antibody (E-2) FITC | sc-515540 FITC | 200 µg/ml | $330.00 | |||
ORP-7 Antibody (E-2) PE | sc-515540 PE | 200 µg/ml | $343.00 | |||
ORP-7 Antibody (E-2) Alexa Fluor® 488 | sc-515540 AF488 | 200 µg/ml | $357.00 | |||
ORP-7 Antibody (E-2) Alexa Fluor® 546 | sc-515540 AF546 | 200 µg/ml | $357.00 | |||
ORP-7 Antibody (E-2) Alexa Fluor® 594 | sc-515540 AF594 | 200 µg/ml | $357.00 | |||
ORP-7 Antibody (E-2) Alexa Fluor® 647 | sc-515540 AF647 | 200 µg/ml | $357.00 | |||
ORP-7 Antibody (E-2) Alexa Fluor® 680 | sc-515540 AF680 | 200 µg/ml | $357.00 | |||
ORP-7 Antibody (E-2) Alexa Fluor® 790 | sc-515540 AF790 | 200 µg/ml | $357.00 |