ORP-3 Antibody (G-5): sc-514097
- ORP-3 Antibody (G-5) is a mouse monoclonal IgG1 κ ORP-3 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 159-265 mapping within an internal region of ORP-3 of human origin
- ORP-3 Antibody (G-5) is recommended for detection of ORP-3 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-ORP-3 Antibody (G-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ORP-3 Antibody (G-5) for WB applications. These reagents are now offered in bundles with ORP-3 Antibody (G-5) (see ordering information below).
ORP-3 Antibody (G-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects ORP-3 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ORP-3 (G-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ORP-3, also known as OSBP-related protein 3 or OSBPL3, is an 887 amino acid protein that plays a crucial role in regulating cellular processes such as actin cytoskeletal control, cell polarity, and cell adhesion. ORP-3 contains a pleckstrin homology (PH) domain, which is essential for interaction with phosphoinositides, thereby influencing membrane dynamics and signaling pathways. ORP-3 exists as eight alternatively spliced isoforms, designated 1a-1d and 2a-2d, and is expressed in various tissues, including colon, lung, spleen, brain, thyroid, bone marrow, and skeletal muscle, while being absent in liver and heart tissue. ORP-3 is encoded on human chromosome 7, a region associated with several genetic disorders, highlighting ORP-3′s significance in both normal physiology and disease states.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ORP-3 Antibody (G-5) References:
- Family of human oxysterol binding protein (OSBP) homologues. A novel member implicated in brain sterol metabolism. | Laitinen, S., et al. 1999. J Lipid Res. 40: 2204-11. PMID: 10588946
- The OSBP-related protein family in humans. | Lehto, M., et al. 2001. J Lipid Res. 42: 1203-13. PMID: 11483621
- A family of 12 human genes containing oxysterol-binding domains. | Jaworski, CJ., et al. 2001. Genomics. 78: 185-96. PMID: 11735225
- ORP3 splice variants and their expression in human tissues and hematopoietic cells. | Collier, FM., et al. 2003. DNA Cell Biol. 22: 1-9. PMID: 12590732
- Subfamily III of mammalian oxysterol-binding protein (OSBP) homologues: the expression and intracellular localization of ORP3, ORP6, and ORP7. | Lehto, M., et al. 2004. Cell Tissue Res. 315: 39-57. PMID: 14593528
- Targeting of OSBP-related protein 3 (ORP3) to endoplasmic reticulum and plasma membrane is controlled by multiple determinants. | Lehto, M., et al. 2005. Exp Cell Res. 310: 445-62. PMID: 16143324
- The R-Ras interaction partner ORP3 regulates cell adhesion. | Lehto, M., et al. 2008. J Cell Sci. 121: 695-705. PMID: 18270267
- Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. | Ishikawa, K., et al. 1998. DNA Res. 5: 169-76. PMID: 9734811
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ORP-3 Antibody (G-5) | sc-514097 | 200 µg/ml | $322.00 | |||
ORP-3 Antibody (G-5): m-IgG Fc BP-HRP Bundle | sc-531029 | 200 µg Ab; 10 µg BP | $361.00 | |||
ORP-3 Antibody (G-5): m-IgGκ BP-HRP Bundle | sc-524604 | 200 µg Ab, 40 µg BP | $361.00 | |||
ORP-3 Antibody (G-5): m-IgG1 BP-HRP Bundle | sc-544365 | 200 µg Ab; 20 µg BP | $361.00 | |||
ORP-3 Antibody (G-5) AC | sc-514097 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ORP-3 Antibody (G-5) HRP | sc-514097 HRP | 200 µg/ml | $322.00 | |||
ORP-3 Antibody (G-5) FITC | sc-514097 FITC | 200 µg/ml | $336.00 | |||
ORP-3 Antibody (G-5) PE | sc-514097 PE | 200 µg/ml | $349.00 | |||
ORP-3 Antibody (G-5) Alexa Fluor® 488 | sc-514097 AF488 | 200 µg/ml | $364.00 | |||
ORP-3 Antibody (G-5) Alexa Fluor® 546 | sc-514097 AF546 | 200 µg/ml | $364.00 | |||
ORP-3 Antibody (G-5) Alexa Fluor® 594 | sc-514097 AF594 | 200 µg/ml | $364.00 | |||
ORP-3 Antibody (G-5) Alexa Fluor® 647 | sc-514097 AF647 | 200 µg/ml | $364.00 | |||
ORP-3 Antibody (G-5) Alexa Fluor® 680 | sc-514097 AF680 | 200 µg/ml | $364.00 | |||
ORP-3 Antibody (G-5) Alexa Fluor® 790 | sc-514097 AF790 | 200 µg/ml | $364.00 |