ORC4 Antibody (H-2): sc-398455
- ORC4 Antibody (H-2) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 332-347 within an internal region of ORC4 of human origin
- Anti-ORC4 Antibody (H-2) is recommended for detection of ORC4 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-ORC4 Antibody (H-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-398455 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ORC4 Antibody (H-2) for WB applications. These reagents are now offered in bundles with ORC4 Antibody (H-2) (see ordering information below).
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ORC4 Antibody (H-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects ORC4 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ORC4 (H-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ORC4 is a vital component of the origin recognition complex (ORC), which is essential for the initiation of DNA replication. ORC4 plays a critical role in forming pre-replication complexes necessary for accurate and timely DNA replication, ensuring genomic stability during cell division. ORC4, along with other ORC subunits, binds to specific origins of replication in the cell nucleus, serving as a scaffold for the assembly of additional initiation factors. Proper functioning of ORC4 is crucial for the G1/S transition in the cell cycle, as ORC4 helps regulate DNA replication timing, thereby preventing errors that could lead to genomic instability or cancer. Consistent expression of ORC4 throughout the cell cycle underscores its importance in maintaining cellular integrity and function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ORC4 Antibody (H-2) References:
- Selective instability of Orc1 protein accounts for the absence of functional origin recognition complexes during the M-G(1) transition in mammals. | Natale, DA., et al. 2000. EMBO J. 19: 2728-38. PMID: 10835370
- Identification and characterization of the human ORC6 homolog. | Dhar, SK. and Dutta, A. 2000. J Biol Chem. 275: 34983-8. PMID: 10945994
- Subsets of human origin recognition complex (ORC) subunits are expressed in non-proliferating cells and associate with non-ORC proteins. | Thome, KC., et al. 2000. J Biol Chem. 275: 35233-41. PMID: 10954718
- Chromatin association of human origin recognition complex, cdc6, and minichromosome maintenance proteins during the cell cycle: assembly of prereplication complexes in late mitosis. | Méndez, J. and Stillman, B. 2000. Mol Cell Biol. 20: 8602-12. PMID: 11046155
- Assembly of the human origin recognition complex. | Vashee, S., et al. 2001. J Biol Chem. 276: 26666-73. PMID: 11323433
- Architecture of the human origin recognition complex. | Dhar, SK., et al. 2001. J Biol Chem. 276: 29067-71. PMID: 11395502
- Identification of HsORC4, a member of the human origin of replication recognition complex. | Quintana, DG., et al. 1997. J Biol Chem. 272: 28247-51. PMID: 9353276
- The human origin recognition complex protein 1 dissociates from chromatin during S phase in HeLa cells. | Kreitz, S., et al. 2001. J Biol Chem. 276: 6337-42. PMID: 11102449
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ORC4 Antibody (H-2) | sc-398455 | 200 µg/ml | $316.00 | |||
ORC4 Antibody (H-2): m-IgG Fc BP-HRP Bundle | sc-530805 | 200 µg Ab; 10 µg BP | $354.00 | |||
ORC4 Antibody (H-2): m-IgGκ BP-HRP Bundle | sc-524349 | 200 µg Ab, 40 µg BP | $354.00 | |||
ORC4 Antibody (H-2) X | sc-398455 X | 200 µg/0.1 ml | $316.00 | |||
ORC4 Antibody (H-2) AC | sc-398455 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ORC4 Antibody (H-2) HRP | sc-398455 HRP | 200 µg/ml | $316.00 | |||
ORC4 Antibody (H-2) FITC | sc-398455 FITC | 200 µg/ml | $330.00 | |||
ORC4 Antibody (H-2) PE | sc-398455 PE | 200 µg/ml | $343.00 | |||
ORC4 Antibody (H-2) Alexa Fluor® 488 | sc-398455 AF488 | 200 µg/ml | $357.00 | |||
ORC4 Antibody (H-2) Alexa Fluor® 546 | sc-398455 AF546 | 200 µg/ml | $357.00 | |||
ORC4 Antibody (H-2) Alexa Fluor® 594 | sc-398455 AF594 | 200 µg/ml | $357.00 | |||
ORC4 Antibody (H-2) Alexa Fluor® 647 | sc-398455 AF647 | 200 µg/ml | $357.00 | |||
ORC4 Antibody (H-2) Alexa Fluor® 680 | sc-398455 AF680 | 200 µg/ml | $357.00 | |||
ORC4 Antibody (H-2) Alexa Fluor® 790 | sc-398455 AF790 | 200 µg/ml | $357.00 | |||
ORC4 (H-2) Neutralizing Peptide | sc-398455 P | 100 µg/0.5 ml | $68.00 |