ORC3 Antibody (1D6): sc-23888

ORC3 Antibody (1D6) is a rat monoclonal IgG2b antibody that detects ORC3 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-ORC3 antibody (1D6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ORC3 is a vital component of the origin recognition complex (ORC), which is essential for the initiation of DNA replication. ORC3 plays a critical role in forming pre-replication complexes necessary for accurate and timely DNA replication during the cell cycle. ORC3, along with other ORC subunits, binds to specific origins of replication, serving as a scaffold for the assembly of additional initiation factors. Proper functioning of ORC3 is crucial for G1-S transition, ensuring DNA replication occurs only once per cell cycle, thereby maintaining genomic stability. Dysregulation of ORC proteins, including ORC3, can lead to replication stress and has been implicated in various diseases, including cancer. Understanding ORC3′s role in the cell cycle and interactions with other ORC subunits is essential for elucidating DNA replication mechanisms and implications in cellular health and disease.

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ORC3 Antibody (1D6) References:

1. Identification and chromosomal localization of murine ORC3, a new member of the mouse origin recognition complex.  |  Springer, J., et al. 1999. Cytogenet Cell Genet. 87: 245-51. PMID: 10702681
2. Selective instability of Orc1 protein accounts for the absence of functional origin recognition complexes during the M-G(1) transition in mammals.  |  Natale, DA., et al. 2000. EMBO J. 19: 2728-38. PMID: 10835370
3. Identification and characterization of the human ORC6 homolog.  |  Dhar, SK. and Dutta, A. 2000. J Biol Chem. 275: 34983-8. PMID: 10945994
4. Subsets of human origin recognition complex (ORC) subunits are expressed in non-proliferating cells and associate with non-ORC proteins.  |  Thome, KC., et al. 2000. J Biol Chem. 275: 35233-41. PMID: 10954718
5. Chromatin association of human origin recognition complex, cdc6, and minichromosome maintenance proteins during the cell cycle: assembly of prereplication complexes in late mitosis.  |  Méndez, J. and Stillman, B. 2000. Mol Cell Biol. 20: 8602-12. PMID: 11046155
6. Assembly of the human origin recognition complex.  |  Vashee, S., et al. 2001. J Biol Chem. 276: 26666-73. PMID: 11323433
7. Architecture of the human origin recognition complex.  |  Dhar, SK., et al. 2001. J Biol Chem. 276: 29067-71. PMID: 11395502
8. The origin recognition complex subunit, ORC3, is developmentally regulated and supports the expression of biochemical markers of neuronal maturation in cultured cerebellar granule cells.  |  Cappuccio, I., et al. 2010. Brain Res. 1358: 1-10. PMID: 20674557
9. Study of the Interaction between Xmas-2, the Main Protein of TREX-2 mRNA Export Complex, and the Orc3 Protein, a Subunit of ORC Complex of D. melanogaster.  |  Kurshakova, MM., et al. 2021. Dokl Biochem Biophys. 496: 18-21. PMID: 33689068
10. Stability of proteins involved in initiation of DNA replication in UV damaged human cells.  |  Anachkova, BB. and Djeliova, VL. 2022. Z Naturforsch C J Biosci. 77: 113-123. PMID: 34333892
11. Identification of HsORC4, a member of the human origin of replication recognition complex.  |  Quintana, DG., et al. 1997. J Biol Chem. 272: 28247-51. PMID: 9353276
12. The human origin recognition complex protein 1 dissociates from chromatin during S phase in HeLa cells.  |  Kreitz, S., et al. 2001. J Biol Chem. 276: 6337-42. PMID: 11102449

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ORC3 Antibody (1D6) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-ORC3 Antibody (1D6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ORC3 Antibody (1D6) Specifications

Alternate Product Name: ORC3

Product Clone Name: 1D6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b

Amino Acids:

Epitope Target: ORC3

Epitope Species: human

Product Reactivity/Detection: ORC3

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2b in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for ORC3 Antibody (1D6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred ORC3 antibody is ORC3 Antibody (C-12): sc-374231.