Date published: 2026-4-3

1-800-457-3801

SCBT Portrait Logo
Seach Input

osteopontin/OPN/SPP1 Antibody (LFMb-14): sc-73631

4.7(7)
Write a reviewAsk a question

Datasheets
  • osteopontin/OPN/SPP1 Antibody (LFMb-14) is a mouse monoclonal IgG2b κ, cited in 62 publications, provided at 200 µg/ml
  • raised against full length recombinant OPN of human origin
  • Anti-osteopontin/OPN/SPP1 Antibody (LFMb-14) is recommended for detection of OPN of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
  • Anti-osteopontin/OPN/SPP1 Antibody (LFMb-14) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP and m-IgG2b BP-HRP are the preferred secondary detection reagents for osteopontin/OPN/SPP1 Antibody (LFMb-14) for WB and IHC(P) applications. These reagents are now offered in bundles with osteopontin/OPN/SPP1 Antibody (LFMb-14) (see ordering information below).
Gene Editing Promo Banner

QUICK LINKS

SEE ALSO...

OPN Antibody (LFMb-14) is a mouse monoclonal IgG2b kappa light chain antibody that detects osteopontin (OPN) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Anti-OPN antibody (LFMb-14) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Osteopontin (OPN), also known as bone sialoprotein 1, urinary stone protein, SPP1, Eta-1, nephropontin, and uropontin, is a crucial extracellular matrix cell adhesion phosphoglycoprotein that plays a significant role in various biological processes. One of the key aspects of OPN is post-translational modifications, which can lead to variations in molecular weight and functional properties. These modifications, such as phosphorylation and glycosylation, are essential for regulating OPN′s interactions with other proteins and biological activities, including cell adhesion, migration, and immune response modulation. Post-translational modifications allow OPN to participate in diverse physiological and pathological processes, including tissue remodeling, wound healing, and tumor progression, making OPN a vital protein in both health and disease.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

osteopontin/OPN/SPP1 Antibody (LFMb-14)

sc-73631
200 µg/ml
$322.00

osteopontin/OPN/SPP1 Antibody (LFMb-14): m-IgG Fc BP-HRP Bundle

sc-525701
200 µg Ab; 10 µg BP
$361.00

osteopontin/OPN/SPP1 Antibody (LFMb-14): m-IgG2b BP-HRP Bundle

sc-549626
200 µg Ab; 10 µg BP
$361.00

osteopontin/OPN/SPP1 Antibody (LFMb-14) AC

sc-73631 AC
500 µg/ml, 25% agarose
$424.00

osteopontin/OPN/SPP1 Antibody (LFMb-14) HRP

sc-73631 HRP
200 µg/ml
$322.00

osteopontin/OPN/SPP1 Antibody (LFMb-14) FITC

sc-73631 FITC
200 µg/ml
$336.00

osteopontin/OPN/SPP1 Antibody (LFMb-14) PE

sc-73631 PE
200 µg/ml
$349.00

osteopontin/OPN/SPP1 Antibody (LFMb-14) Alexa Fluor® 488

sc-73631 AF488
200 µg/ml
$364.00

osteopontin/OPN/SPP1 Antibody (LFMb-14) Alexa Fluor® 546

sc-73631 AF546
200 µg/ml
$364.00

osteopontin/OPN/SPP1 Antibody (LFMb-14) Alexa Fluor® 594

sc-73631 AF594
200 µg/ml
$364.00

osteopontin/OPN/SPP1 Antibody (LFMb-14) Alexa Fluor® 647

sc-73631 AF647
200 µg/ml
$364.00

osteopontin/OPN/SPP1 Antibody (LFMb-14) Alexa Fluor® 680

sc-73631 AF680
200 µg/ml
$364.00

osteopontin/OPN/SPP1 Antibody (LFMb-14) Alexa Fluor® 790

sc-73631 AF790
200 µg/ml
$364.00

Is it recommended for WB and IHC in mouse liver?

Asked by: Bea31
Thank you for your question. Anti-osteopontin/OPN/SPP1 Antibody (LFMb-14) is recommended for detection of OPN of various cell and tissue types from mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM and will also work on liver samples.
Answered by: Tech Support Europe
Date published: 2022-06-13

Is OPN Antibody (LFMb-14): sc-73631 available directly conjugated to HRP or fluorescent dyes?

Asked by: jenni
Thank you for your question. All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at specialorders@scbt.com Alternatively, consider using OPN (AKm2A1), which is available conjugated to agarose, HRP, FITC, PE, and more. (https://www.scbt.com/scbt/product/opn-antibody-akm2a1)
Answered by: Technical Support 6
Date published: 2017-01-09
  • y_2026, m_3, d_26, h_7CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.43
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_en_US, sid_73631, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 98ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Great productStrong western blot data; also nice IHC staining !!
Date published: 2017-01-10
Rated 5 out of 5 by from Especially good for IHCThis antibody is well-cited for a variety of applications but is especially good in IHC. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2016-12-22
Rated 5 out of 5 by from Used on a regular basis and published as wellUsed on a regular basis and published as well. They are working great for us.
Date published: 2015-05-28
Rated 4 out of 5 by from IHC: pfa fixed murine kidney samplesIHC: pfa fixed murine kidney samples. -SCBT Publication Review
Date published: 2015-04-03
Rated 4 out of 5 by from Good specificity shown by using transfectedGood specificity shown by using transfected and non-transfected 293T cells for over-expression of the OPN protein via Western Blot analysis. -SCBT QC
Date published: 2015-02-04
Rated 5 out of 5 by from Clean bands in western blot analysis of OPNClean bands in western blot analysis of OPN expression in HOS, U-87 MG and Caki-1 whole cell lysates. -SCBT QC
Date published: 2015-01-29
Rated 5 out of 5 by from Worked well for IHC and WB with NTera2Worked well for IHC and WB with NTera2, 2102Ep and TCam-2 cell line. -SCBT Publication Review
Date published: 2015-01-29
  • y_2026, m_3, d_26, h_7
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.43
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_7
  • loc_en_US, sid_73631, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 9ms
  • REVIEWS, PRODUCT
osteopontin/OPN/SPP1 Antibody (LFMb-14) is rated 4.7 out of 5 by 7.
  • y_2026, m_3, d_26, h_7
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.43
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_7
  • loc_en_US, sid_73631, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 126ms
  • REVIEWS, PRODUCT