OPLAH Antibody (H-10): sc-398205

OPLAH Antibody (H-10) is a mouse monoclonal IgG2a antibody that detects OPLAH in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). OPLAH, also known as 5-oxoprolinase (ATP-hydrolysing), plays a critical role in the gamma-glutamyl cycle, which is essential for the synthesis and metabolism of glutathione, a key antioxidant that regulates amino acid transport and protects cells from oxidative stress. This enzyme catalyzes the conversion of 5-oxo-L-proline to L-glutamate, a reaction that is coupled with the hydrolysis of ATP, highlighting its importance in energy metabolism and cellular function. OPLAH is a 1,288 amino acid protein that forms a homodimer and belongs to the oxoprolinase family, with highest expression levels found in the kidney, and lower levels in the lung, breast, colon, and ovary. The gene encoding OPLAH is located on human chromosome 8, which is associated with various diseases, including schizophrenia and congenital hypothyroidism, underscoring OPLAH′s potential relevance in both health and disease. Anti-OPLAH antibody (H-10) is an invaluable tool for researchers studying the gamma-glutamyl cycle and its implications in cellular metabolism and disease mechanisms.

The preferred OPLAH antibody is OPLAH Antibody (E-10): sc-271807.