OPLAH Antibody (E-10): sc-271807
- OPLAH Antibody (E-10) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 27-57 near the N-terminus of OPLAH of human origin
- Anti-OPLAH Antibody (E-10) is recommended for detection of OPLAH of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including canine
- Anti-OPLAH Antibody (E-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for OPLAH Antibody (E-10). This work is in progress.
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SEE ALSO...
OPLAH Antibody (E-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects OPLAH in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-OPLAH antibody (E-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. OPLAH, also known as 5-oxoprolinase (ATP-hydrolysing), plays a critical role in the gamma-glutamyl cycle, which is essential for the synthesis and metabolism of glutathione, a key antioxidant that regulates amino acid transport and protects cells from oxidative stress. This enzyme catalyzes the conversion of 5-oxo-L-proline to L-glutamate, a reaction that is coupled with ATP hydrolysis, highlighting OPLAH′s importance in cellular energy metabolism and amino acid homeostasis. OPLAH is a 1,288 amino acid protein that functions as a homodimer and is predominantly expressed in the kidney, with lower expression levels found in the lung, breast, colon, and ovary. The gene encoding OPLAH is located on human chromosome 8, which is associated with various diseases, including schizophrenia, bipolar disorder, and congenital hypothyroidism, underscoring OPLAH′s potential relevance in both health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
OPLAH Antibody (E-10) References:
- Localization of 5-oxo-L-prolinase mRNA in the murine choroid plexus by in situ hybridization. | Jäger, M. and Wolf, S. 1999. Neurosci Lett. 274: 171-4. PMID: 10548417
- Preferential expression of an intact WRN gene in Werner syndrome cell lines in which a normal chromosome 8 has been introduced. | Kashino, G., et al. 2001. Biochem Biophys Res Commun. 289: 111-5. PMID: 11708785
- Cytogenetic mapping of a novel locus for type II Waardenburg syndrome. | Selicorni, A., et al. 2002. Hum Genet. 110: 64-7. PMID: 11810298
- Bovine 5-oxo-L-prolinase: simple assay method, purification, cDNA cloning, and detection of mRNA in the coronary artery. | Watanabe, T., et al. 2004. Biol Pharm Bull. 27: 288-94. PMID: 14993790
- Combined analysis from eleven linkage studies of bipolar disorder provides strong evidence of susceptibility loci on chromosomes 6q and 8q. | McQueen, MB., et al. 2005. Am J Hum Genet. 77: 582-95. PMID: 16175504
- Glutamine affects glutathione recycling enzymes in a DMBA-induced breast cancer model. | Kaufmann, Y., et al. 2008. Nutr Cancer. 60: 518-25. PMID: 18584486
- Activity and distribution of the cysteine prodrug activating enzyme, 5-oxo-L-prolinase, in human normal and tumor tissues. | Srivenugopal, KS. and Ali-Osman, F. 1997. Cancer Lett. 117: 105-11. PMID: 9233839
- Characterization of 5-oxo-L-prolinase in normal and tumor tissues of humans and rats: a potential new target for biochemical modulation of glutathione. | Chen, X., et al. 1998. Clin Cancer Res. 4: 131-8. PMID: 9516961
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OPLAH Antibody (E-10) | sc-271807 | 200 µg/ml | $322.00 | |||
OPLAH Antibody (E-10) AC | sc-271807 AC | 500 µg/ml, 25% agarose | $424.00 | |||
OPLAH Antibody (E-10) HRP | sc-271807 HRP | 200 µg/ml | $322.00 | |||
OPLAH Antibody (E-10) FITC | sc-271807 FITC | 200 µg/ml | $336.00 | |||
OPLAH Antibody (E-10) PE | sc-271807 PE | 200 µg/ml | $349.00 | |||
OPLAH Antibody (E-10) Alexa Fluor® 488 | sc-271807 AF488 | 200 µg/ml | $364.00 | |||
OPLAH Antibody (E-10) Alexa Fluor® 546 | sc-271807 AF546 | 200 µg/ml | $364.00 | |||
OPLAH Antibody (E-10) Alexa Fluor® 594 | sc-271807 AF594 | 200 µg/ml | $364.00 | |||
OPLAH Antibody (E-10) Alexa Fluor® 647 | sc-271807 AF647 | 200 µg/ml | $364.00 | |||
OPLAH Antibody (E-10) Alexa Fluor® 680 | sc-271807 AF680 | 200 µg/ml | $364.00 | |||
OPLAH Antibody (E-10) Alexa Fluor® 790 | sc-271807 AF790 | 200 µg/ml | $364.00 | |||
OPLAH (E-10) Neutralizing Peptide | sc-271807 P | 100 µg/0.5 ml | $69.00 |