Odf1 CRISPR/Cas9 KO Plasmid (h): sc-405840
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Odf1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Odf1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Odf1 Antibody (E-11): sc-390152
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Odf1 CRISPR/Cas9 KO Plasmid (h) | sc-405840 | 20 µg | $397.00 |
Overview
ODF1 encodes outer dense fiber protein 1 (Odf1), a testis-enriched structural component of sperm tail outer dense fibers that supports axonemal stability and elastic recoil during flagellar beating. Odf1 participates in spermiogenesis and late-stage sperm assembly, contributing to cytoskeletal organization and mechanical integrity of the principal piece. Disruption of ODF1-dependent sperm tail architecture is linked to impaired motility and male factor infertility phenotypes, making it relevant for studying mechanisms of asthenozoospermia and related spermatogenic defects. Its restricted expression profile and specialized function provide a focused model for investigating differentiation-dependent regulation of cytoskeletal proteins in germ cells.
Odf1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ODF1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ODF1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ODF1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Odf1 protein expression.
This CRISPR knockout system enables efficient generation of ODF1-deficient cell models for investigation of Odf1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ODF1 exon(s) critical for Odf1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ODF1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Odf1 CRISPR/Cas9 KO Plasmid (h) and Odf1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ODF1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Odf1 HDR Plasmid (h) and Odf1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ODF1 homology arms to support homology-directed repair at defined ODF1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.