OctA-Probe Antibody (H-5): sc-166355

OctA-Probe Antibody (H-5) is a mouse monoclonal IgG1 kappa light chain antibody engineered to detect OctA, also known as the FLAG tag or DYKDDDDK peptide, which is frequently fused to proteins of interest for various research applications. anti-OctA antibody (H-5) is effective in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA), providing researchers with versatile tools for protein detection and analysis. The FLAG tag is typically incorporated into the N-terminus of proteins expressed in bacterial, insect, and mammalian systems, facilitating the purification and visualization of target proteins without significantly disrupting their native functions. Depending on the fusion protein, the FLAG tag can be located in the cytoplasm, nucleus, or other cellular compartments, allowing precise tracking and study of protein localization and interactions within the cell. This spatial information is crucial for understanding the biological roles and mechanisms of the proteins under investigation. OctA-Probe (H-5) monoclonal antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, catering to a wide range of experimental needs. The small, hydrophilic FLAG peptide ensures that fused proteins retain their biological activity and structural integrity, while the tag can be removed post-purification using enterokinase for further functional studies. OctA-Probe (H-5) antibody supports advanced research in protein characterization, interaction mapping, and cellular localization through reliable detection and purification of FLAG-tagged proteins.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

OctA-Probe Antibody (H-5) References:

1. Evidence for direct interaction between Sprouty and Cbl.  |  Wong, ES., et al. 2001. J Biol Chem. 276: 5866-75. PMID: 11053437
2. alpha 2 integrin subunit cytoplasmic domain-dependent cellular migration requires p38 MAPK.  |  Klekotka, PA., et al. 2001. J Biol Chem. 276: 9503-11. PMID: 11121402
3. Transgenic mice with an expanded CAG repeat controlled by the human AR promoter show polyglutamine nuclear inclusions and neuronal dysfunction without neuronal cell death.  |  Adachi, H., et al. 2001. Hum Mol Genet. 10: 1039-48. PMID: 11331614
4. Structural domains required for channel function of the mouse transient receptor potential protein homologue TRP1beta.  |  Engelke, M., et al. 2002. FEBS Lett. 523: 193-9. PMID: 12123831
5. Regulation of ania-6 splice variants by distinct signaling pathways in striatal neurons.  |  Sgambato, V., et al. 2003. J Neurochem. 86: 153-64. PMID: 12807435
6. The differentiation-dependent desmosomal cadherin desmoglein 1 is a novel caspase-3 target that regulates apoptosis in keratinocytes.  |  Dusek, RL., et al. 2006. J Biol Chem. 281: 3614-24. PMID: 16286477
7. Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin.  |  Itakura, K., et al. 1977. Science. 198: 1056-63. PMID: 412251
8. Expression in Escherichia coli of chemically synthesized genes for human insulin.  |  Goeddel, DV., et al. 1979. Proc Natl Acad Sci U S A. 76: 106-10. PMID: 85300

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-OctA-Probe Antibody (H-5) has 111 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

OctA-Probe Antibody (H-5) Specifications

Alternate Product Name: FLAG tag

Product Clone Name: H-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: OctA-Probe

Epitope Species:

Product Reactivity/Detection: OctA (FLAG®)-tagged fusion proteins

Species Reactivity/Detection: OctA, FLAG tagged proteins

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 111 citations in various scientific publications

Available Conjugates/Forms for OctA-Probe Antibody (H-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred OctA-Probe antibody is OctA-Probe Antibody (G-8): sc-166384.