OctA-Probe Antibody (G-8): sc-166384

OctA-Probe Antibody (G-8) is a mouse monoclonal IgG2b kappa light chain antibody engineered to recognize OctA, also known as the FLAG tag or DYKDDDDK peptide, which is widely used in protein research. anti-OctA antibody (G-8) effectively detects FLAG-tagged fusion proteins in applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA), providing versatile tools for diverse experimental needs. The FLAG tag is an eight-amino-acid hydrophilic peptide incorporated into the N-terminus of target proteins expressed in bacterial, insect, and mammalian systems, facilitating their purification and visualization without significantly altering their native structure or function. The compact and hydrophilic nature of the FLAG tag ensures that fused proteins retain their biological activity and structural integrity, which is essential for accurate functional studies and interaction analyses. Additionally, the FLAG tag enhances solubility and simplifies the purification process, making it easier to isolate proteins of interest. OctA-Probe (G-8) monoclonal antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, allowing researchers to choose the appropriate format for their specific applications. The FLAG tag can be removed from the target protein post-purification using enterokinase, enabling further functional analyses without residual tags. OctA-Probe (G-8) antibody supports advanced studies in protein characterization, interaction mapping, and cellular localization by providing reliable detection and minimal disruption to protein structure.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-OctA-Probe Antibody (G-8) has 19 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

OctA-Probe Antibody (G-8) Specifications

Alternate Product Name: FLAG tag

Product Clone Name: G-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids:

Epitope Target: OctA-Probe

Epitope Species:

Product Reactivity/Detection: OctA (FLAG®)-tagged fusion proteins

Species Reactivity/Detection: OctA, FLAG tagged proteins

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 19 citations in various scientific publications

Available Conjugates/Forms for OctA-Probe Antibody (G-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred OctA-Probe antibody is OctA-Probe Antibody (H-5): sc-166355.