OctA-Probe Antibody (F-tag-01): sc-51590

OctA-Probe Antibody (F-tag-01) is a mouse monoclonal IgG1 kappa light chain antibody specifically designed to recognize the OctA epitope, also known as the FLAG tag, with the amino acid sequence DYKDDDDK. OctA-Probe monoclonal antibody (F-tag-01) effectively detects FLAG-tagged fusion proteins across various applications, including western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). The FLAG tag, a short hydrophilic peptide, is commonly engineered into the N-terminus of proteins expressed in bacterial, insect, and mammalian systems, facilitating the purification and visualization of target proteins without significantly disrupting their biological activity. By providing a reliable means to isolate and study proteins of interest, the FLAG tag plays a crucial role in advancing research in protein function and interaction studies. OctA-Probe (F-tag-01) antibody is available in a non-conjugated form, allowing flexibility for researchers to use in conjunction with various secondary detection systems. Additionally, the FLAG tag can be removed post-purification through cleavage with enterokinase, offering further versatility in experimental designs. The ability to maintain protein functionality while enabling precise detection underscores the importance of the FLAG tag system in molecular biology. With its proven utility in enhancing protein analysis workflows, OctA-Probe (F-tag-01) monoclonal antibody is an essential tool for researchers aiming to achieve accurate and efficient protein characterization.

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-OctA-Probe Antibody (F-tag-01) has 19 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

OctA-Probe Antibody (F-tag-01) Specifications

Alternate Product Name: FLAG tag

Product Clone Name: F-tag-01

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: OctA-Probe

Epitope Species:

Product Reactivity/Detection: OctA (FLAG®)-tagged fusion proteins

Species Reactivity/Detection:

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 19 citations in various scientific publications

Available Conjugates/Forms for OctA-Probe Antibody (F-tag-01): N/A

The preferred OctA-Probe antibody is OctA-Probe Antibody (H-5): sc-166355.