Ob-R/Leptin Receptor Antibody (B-3): sc-8391
- Ob-R/Leptin Receptor Antibody (B-3) is a mouse monoclonal IgG1 κ Ob-R/Leptin Receptor antibody, cited in 113 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 870-894 of the short form of Ob-R of mouse origin
- Ob-R/Leptin Receptor Antibody (B-3) is recommended for detection of short and long forms of Ob-R of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine
- Anti-Ob-R/Leptin Receptor Antibody (B-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Ob-R/Leptin Receptor Antibody (B-3) for WB and IHC(P) applications. These reagents are now offered in bundles with Ob-R/Leptin Receptor Antibody (B-3) (see ordering information below).
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Ob-R Antibody (B-3) is a mouse monoclonal IgG1 kappa light chain antibody that specifically targets an epitope between amino acids 870-894 of the short form of the leptin receptor (Ob-R) of mouse origin. Ob-R monoclonal antibody (B-3) detects both the short and long forms of Ob-R, also known as LEPR or CD295, in species including mouse, rat, human, and canine, making Ob-R (B-3) antibody suitable for applications like western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). The Ob-R leptin receptor is a member of the class I cytokine receptor family and structurally resembles the gp130 signal-transducing component of the IL-6 receptor complex. Ob-R plays a critical role in regulating body weight, energy balance, and neuroendocrine functions by mediating the effects of leptin, a hormone produced by adipose tissue that suppresses appetite and promotes energy expenditure. Predominantly expressed in the hypothalamus and choroid plexus, Ob-R is pivotal in signaling pathways that control food intake and body weight homeostasis. Dysregulation or mutations in the leptin receptor can lead to obesity and metabolic disorders, including type II diabetes, making Ob-R a significant target for research in metabolic regulation and energy homeostasis. Ob-R monoclonal antibody (B-3) facilitates advanced studies on leptin signaling pathways and their implications in metabolic and neuroendocrine functions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ob-R/Leptin Receptor Antibody (B-3) References:
- Tackling a weighty problem. | Friedman, JM. and Leibel, RL. 1992. Cell. 69: 217-20. PMID: 1568242
- Molecular mapping of the mouse ob mutation. | Friedman, JM., et al. 1991. Genomics. 11: 1054-62. PMID: 1686014
- Leptin/OB-R signaling is elevated in mice with Sjögren's syndrome and is implicated in disease pathogenesis. | Xu, T., et al. 2017. Biochem Biophys Res Commun. 482: 835-842. PMID: 27889607
- Leptin/OB-R pathway promotes IL-4 secretion from B lymphocytes and induces salivary gland epithelial cell apoptosis in Sjögren's syndrome. | Xu, T., et al. 2017. Oncotarget. 8: 63417-63429. PMID: 28969001
- Predictive Role of Leptin Receptor (Ob-R) Overexpression in Patients with Early Breast Cancer Receiving Neoadjuvant Systemic Treatment. | García-Estévez, L., et al. 2021. Cancers (Basel). 13: PMID: 34210055
- Genetics. In search of a satiety factor. | Rink, TJ. 1994. Nature. 372: 406-7. PMID: 7984230
- Positional cloning of the mouse obese gene and its human homologue. | Zhang, Y., et al. 1994. Nature. 372: 425-32. PMID: 7984236
- Leptin and OB-R: body weight regulation by a cytokine receptor. | White, DW. and Tartaglia, LA. 1996. Cytokine Growth Factor Rev. 7: 303-9. PMID: 9023054
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ob-R/Leptin Receptor Antibody (B-3) | sc-8391 | 200 µg/ml | $316.00 | |||
Ob-R/Leptin Receptor Antibody (B-3): m-IgG Fc BP-HRP Bundle | sc-526514 | 200 µg Ab; 10 µg BP | $354.00 | |||
Ob-R/Leptin Receptor Antibody (B-3): m-IgG1 BP-HRP Bundle | sc-531887 | 200 µg Ab; 20 µg BP | $354.00 | |||
Ob-R/Leptin Receptor Antibody (B-3) AC | sc-8391 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Ob-R/Leptin Receptor Antibody (B-3) HRP | sc-8391 HRP | 200 µg/ml | $316.00 | |||
Ob-R/Leptin Receptor Antibody (B-3) FITC | sc-8391 FITC | 200 µg/ml | $330.00 | |||
Ob-R/Leptin Receptor Antibody (B-3) PE | sc-8391 PE | 200 µg/ml | $343.00 | |||
Ob-R/Leptin Receptor Antibody (B-3) Alexa Fluor® 488 | sc-8391 AF488 | 200 µg/ml | $357.00 | |||
Ob-R/Leptin Receptor Antibody (B-3) Alexa Fluor® 546 | sc-8391 AF546 | 200 µg/ml | $357.00 | |||
Ob-R/Leptin Receptor Antibody (B-3) Alexa Fluor® 594 | sc-8391 AF594 | 200 µg/ml | $357.00 | |||
Ob-R/Leptin Receptor Antibody (B-3) Alexa Fluor® 647 | sc-8391 AF647 | 200 µg/ml | $357.00 | |||
Ob-R/Leptin Receptor Antibody (B-3) Alexa Fluor® 680 | sc-8391 AF680 | 200 µg/ml | $357.00 | |||
Ob-R/Leptin Receptor Antibody (B-3) Alexa Fluor® 790 | sc-8391 AF790 | 200 µg/ml | $357.00 | |||
Ob-R/Leptin Receptor (B-3) Neutralizing Peptide | sc-8391 P | 100 µg/0.5 ml | $68.00 |