Date published: 2026-4-5
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Ob Antibody (F-3) - Immunohistochemistry - Image 293336
Ob Antibody (F-3): sc-48408. Immunoperoxidase staining of formalin fixed, paraffin-embedded human ovary tissue showing cytoplasmic staining of endothelial cells and staining of plasma in blood vessesls.
Ob Antibody (F-3): sc-48408. Immunoperoxidase staining of formalin fixed, paraffin-embedded human ovary tissue showing cytoplasmic staining of endothelial cells and staining of plasma in blood vessesls.

Ob Antibody (F-3): sc-48408

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Datasheets
  • Ob Antibody (F-3) is a mouse monoclonal IgG2a κ Ob antibody, cited in 4 publications, provided at 200 µg/ml
  • raised against amino acids 92-145 of human Ob
  • Ob Antibody (F-3) is recommended for detection of Ob of human origin by WB, IP, IF, IHC(P) and ELISA
  • Anti-Ob Antibody (F-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Ob Antibody (F-3) for WB and IHC(P) applications. These reagents are now offered in bundles with Ob Antibody (F-3) (see ordering information below).
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SEE ALSO...

Ob Antibody (F-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects Ob protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-Ob antibody (F-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ob protein, also known as leptin, plays a crucial role in regulating energy balance by inhibiting hunger, which is vital for maintaining body weight homeostasis. Leptin is primarily produced by adipose (fat) tissue and acts on receptors in the hypothalamus to signal satiety, thereby influencing food intake and energy expenditure. Dysregulation of leptin signaling is implicated in obesity and metabolic disorders, making Ob protein essential for understanding the molecular mechanisms underlying these conditions. Ob gene encodes a 167 amino acid protein that is highly conserved across species, with an 84% identity between human and mouse sequences, highlighting evolutionary importance. A nonsense mutation in Ob gene has been identified in the original congenic C57BL/6J Ob/Ob mouse strain, which serves as a model for studying obesity and related metabolic diseases.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ob Antibody (F-3) References:

  1. Leptin and undernutrition.  |  Prentice, AM., et al. 2002. Nutr Rev. 60: S56-67; discussion S68-84, 85-7. PMID: 12403085
  2. Leptin and the cardiovascular system.  |  Rahmouni, K. and Haynes, WG. 2004. Recent Prog Horm Res. 59: 225-44. PMID: 14749504
  3. Tackling a weighty problem.  |  Friedman, JM. and Leibel, RL. 1992. Cell. 69: 217-20. PMID: 1568242
  4. Molecular mapping of the mouse ob mutation.  |  Friedman, JM., et al. 1991. Genomics. 11: 1054-62. PMID: 1686014
  5. Leptin and cardiovascular diseases.  |  Hou, N. and Luo, JD. 2011. Clin Exp Pharmacol Physiol. 38: 905-13. PMID: 21957899
  6. Leptin promotes glioblastoma.  |  Lawrence, JE., et al. 2012. Neurol Res Int. 2012: 870807. PMID: 22263109
  7. Leptin and cognition.  |  Warren, MW., et al. 2012. Dement Geriatr Cogn Disord. 33: 410-5. PMID: 22814193
  8. Leptin and reproductive function.  |  Hausman, GJ., et al. 2012. Biochimie. 94: 2075-81. PMID: 22980196
  9. Structural organization and chromosomal assignment of the human obese gene.  |  Isse, N., et al. 1995. J Biol Chem. 270: 27728-33. PMID: 7499240
  10. Genetics. In search of a satiety factor.  |  Rink, TJ. 1994. Nature. 372: 406-7. PMID: 7984230
  11. Positional cloning of the mouse obese gene and its human homologue.  |  Zhang, Y., et al. 1994. Nature. 372: 425-32. PMID: 7984236
  12. Obesity and hypertension.  |  El-Atat, F., et al. 2003. Endocrinol Metab Clin North Am. 32: 823-54. PMID: 14711064

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Ob Antibody (F-3)

sc-48408
200 µg/ml
$322.00

Ob Antibody (F-3): m-IgG Fc BP-HRP Bundle

sc-525563
200 µg Ab; 10 µg BP
$361.00

Ob Antibody (F-3): m-IgG2a BP-HRP Bundle

sc-546316
200 µg Ab; 10 µg BP
$361.00

Ob Antibody (F-3) AC

sc-48408 AC
500 µg/ml, 25% agarose
$424.00

Ob Antibody (F-3) HRP

sc-48408 HRP
200 µg/ml
$322.00

Ob Antibody (F-3) FITC

sc-48408 FITC
200 µg/ml
$336.00

Ob Antibody (F-3) PE

sc-48408 PE
200 µg/ml
$349.00

Ob Antibody (F-3) Alexa Fluor® 488

sc-48408 AF488
200 µg/ml
$364.00

Ob Antibody (F-3) Alexa Fluor® 546

sc-48408 AF546
200 µg/ml
$364.00

Ob Antibody (F-3) Alexa Fluor® 594

sc-48408 AF594
200 µg/ml
$364.00

Ob Antibody (F-3) Alexa Fluor® 647

sc-48408 AF647
200 µg/ml
$364.00

Ob Antibody (F-3) Alexa Fluor® 680

sc-48408 AF680
200 µg/ml
$364.00

Ob Antibody (F-3) Alexa Fluor® 790

sc-48408 AF790
200 µg/ml
$364.00

Hi, I am wondering if the sc-48408 antibody has been used tested on formalin-fixed paraffin-embedded feline samples? What is the expected cross-reactivity between human and cat for the antibody?

Asked by: CeciliaL
Thank you for your question. We have not tested this antibody, Ob (F-3): sc-48408, on feline samples. And we are not aware of any other publications or references from other researchers using the antibody on feline samples. We are not aware of a published protein sequence for Ob or leptin in feline. Please contact our Technical Service Department if you can provide a protein sequence and we can perform sequence analysis with the antigen to predict any potential cross-reactivity with this species. Please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-11-03

Can Ob (F-3): sc-48408 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Ob (F-3): sc-48408 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from buon prodottoho ricevuto una aliquota di prova e l'ho impiegata su tessuti di coniglio con ottimi risultati ad una concentrazione di 1:800
Date published: 2022-06-27
Rated 4 out of 5 by from Ottimo in diluizione 1:50Ho utilizzato l'anticorpo su oociti di diversa specie animale Allego foto di microscopia confocale 40x Protocollo utilizzato: Fissazione in PFA al 4% Pretrattamento in blocking per 4-5 ore (triton-X 0,1%, BSA 0,5%, Glicina 2%, Sodium Azide 0,2 % e Siero 2%) Incubazione primario over night a 4 °C 3 Lavaggi per 30' Incubazione secondario 1:100 antimouse coniugato con alexafluor 488 - 2 ore a 37° 3 lavaggi per 30' . Montaggio in vetrino
Date published: 2021-08-04
Rated 1 out of 5 by from can not detect specific band by WBit can not detect a specific band by WB in our lab.
Date published: 2017-09-18
Rated 1 out of 5 by from IHC Assay Review for Ob mABApplication: Immunohistochemistry. Protocol: Standard protocol for saliva smear staining. Dilutions tested: 1:10, 1:30, 1:90, 1:270. Results: No staining observed.
Date published: 2017-08-31
Rated 5 out of 5 by from Amazing IHC staining!Clean and specific staining on paraffin tissue sections. Really strong and reliable. Great choice!
Date published: 2017-01-25
Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmicProduced beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human ovary tissue. -SCBT QC
Date published: 2015-02-01
Rated 5 out of 5 by from Great Western Blot data of human recombinant ObGreat Western Blot data of human recombinant Ob. -SCBT QC
Date published: 2014-10-09
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Ob Antibody (F-3) is rated 3.7 out of 5 by 7.
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