Nur77 Antibody (E-6) recommended for detection of Nur77 of mouse and rat origin by WB, IP, IF and ELISA

Nur77 Antibody (E-6): sc-166166

Nur77 Antibody (E-6) is rated 5.0 out of 5 by 3.
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Datasheets

    • Nur77 Antibody (E-6) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 59-269 of Nur77 of mouse origin
    • Anti-Nur77 Antibody (E-6) is recommended for detection of Nur77 of mouse and rat origin by WB, IP, IF and ELISA
    • Anti-Nur77 Antibody (E-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-166166 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Nur77 (E-6): sc-166166.
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When should the TransCruz reagent sc-166166 X be used instead of the standard form sc-166166?

Asked by: Randy McDonald
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Excellent! This antibody works great in western blot analysis of Nur77 expression in F9 whole cell lysate.
Date published: 2017-02-08
Rated 5 out of 5 by from Beautiful immunofluorescence nuclear and Beautiful immunofluorescence nuclear and cytoplasmic staining in methanol-fixed NIH/3T3 cells. -SCBT QC
Date published: 2015-02-11
Rated 5 out of 5 by from Satisfactory Western Blot data of Nur77 expression Satisfactory Western Blot data of Nur77 expression in non-transfected and mouse Nur77 transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-11-08
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