Nur77 Antibody (C-5) recommended for detection of Nur77 of mouse, rat and human origin by WB, IP, IF and ELISA

Nur77 Antibody (C-5): sc-365113

Nur77 Antibody (C-5) is rated 5.0 out of 5 by 7.
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Datasheets

    • Anti-Nur77 Antibody (C-5) is a mouse monoclonal IgG1 (kappa light chain) Nur77 antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 4-31 at the N-terminus of Nur77 of human origin
    • Anti-Nur77 Antibody (C-5) is recommended for detection of Nur77 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including bovine
    • Anti-Nur77 Antibody (C-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-365113 X, 200 µg/0.1 ml)
    • blocking peptide, sc-365113 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Nur77 (C-5): sc-365113.
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Hello, is it possible to get a 10µg Gratis sample of Nur 77 (C-5): sc-35511 konjugatet with ether FITC or AF488? Many Thanks! Simone Kumstel

Asked by: SimoneKumstel
Thank you for your question. We do not provide sample sizes of any conjugated primary antibodies. This antibody is available for purchase conjugated to FITC or AF488. Please note that we do offer a 1-year product warranty from the date of shipment for the recommended applications stated on our website. If you require more information, please contact our Technical Service by phone or email or by Live Chat.
Answered by: SCBT Heidelberg Support
Date published: 2018-11-26

WB做不出来,请问你们做出来的一抗稀释比例是多少

Asked by: wuxuan
感谢您的提问。推荐的稀释度是1:100-1:1000,关于此问题的详细细节,欢迎通过:电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”,联系我们的技术支持部门。 Thank you for your inquiry. The recommended dilution for WB is 1:100-1:1000, for more detailed information about this question, please contact our Asian Technical Service team at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 15
Date published: 2018-02-01

What application is the blocking peptide sc-365113 P appropriate for?

Asked by: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Antibody validation in knockout mouse Great antibody - we have confirm its specificity by IF comparing wild type to knockout neuronal tissues. Knockout mouse was purchased from Jackson Lab (Stock No: 006187). Antibody dilution 1/500 in BSA 1%.
Date published: 2017-09-20
Rated 5 out of 5 by from Good antibody! 1:500 diluted for wb of rat protein ,the result is satisfied!
Date published: 2017-05-21
Rated 5 out of 5 by from Works really well for WB Gives a nice single sharp band at around ~65 kD on Western blot. Tested with mouse brain (striatal) lysates.
Date published: 2017-03-15
Rated 5 out of 5 by from Great antibody for Westerns This antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-17
Rated 5 out of 5 by from Good product This antibody was good product. The signal was specific and strong. The dilution is 1:1000 in 5% BSA. I will use this antibody for IF next.
Date published: 2016-12-31
Rated 5 out of 5 by from This antibody works well for IP in cardiac This antibody works well for IP in cardiac fibroblasts.
Date published: 2016-08-15
Rated 5 out of 5 by from Produced nice Western blot data of Nur77 Produced nice Western blot data of Nur77 phosphorylation in non-transfected, untreated human Nur77 transfected and lambda protein phosphatase treated human Nur77 transfected 293 whole cell lysates -SCBT QC
Date published: 2013-05-30
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