NUDC Antibody (C-3): sc-365782

NUDC Antibody (C-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects NUDC protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). NUDC (C-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NUDC, or nuclear distribution gene C homolog, is a highly conserved protein that plays a critical role in cellular processes such as neurogenesis, mitosis, nuclear migration, and cytokinesis. NUDC protein is predominantly located in the cytoplasm and nucleus, where NUDC is essential for proper mitotic spindle formation and chromosome separation during cell division. Notably, NUDC protein undergoes phosphorylation by Polo-like kinase (Plk) at the onset of mitosis, a post-translational modification that is crucial for its function in facilitating accurate cell division and proliferation. Furthermore, in neuronal and fibroblast cells, NUDC protein interacts with LIS1, forming a complex that is vital for the organization of the microtubule network and the transport of nuclei in migrating neurons. This interaction is significant as NUDC also associates with the Dynein/Dynactin motor complex, highlighting the collaborative role of these proteins in regulating cytokinesis and ensuring proper cellular function.

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NUDC Antibody (C-3) References:

1. A homolog of the fungal nuclear migration gene nudC is involved in normal and malignant human hematopoiesis.  |  Miller, BA., et al. 1999. Exp Hematol. 27: 742-50. PMID: 10210332
2. Molecular cloning and characterization of the human NUDC gene.  |  Matsumoto, N. and Ledbetter, DH. 1999. Hum Genet. 104: 498-504. PMID: 10453739
3. Involvement of the fungal nuclear migration gene nudC human homolog in cell proliferation and mitotic spindle formation.  |  Zhang, MY., et al. 2002. Exp Cell Res. 273: 73-84. PMID: 11795948
4. Role for NudC, a dynein-associated nuclear movement protein, in mitosis and cytokinesis.  |  Aumais, JP., et al. 2003. J Cell Sci. 116: 1991-2003. PMID: 12679384
5. A microtubule associated protein (hNUDC) binds to the extracellular domain of thrombopoietin receptor (Mpl).  |  Pan, RM., et al. 2005. J Cell Biochem. 96: 741-50. PMID: 16088917
6. NudC is required for Plk1 targeting to the kinetochore and chromosome congression.  |  Nishino, M., et al. 2006. Curr Biol. 16: 1414-21. PMID: 16860740
7. Regulation of cell differentiation by hNUDC via a Mpl-dependent mechanism in NIH 3T3 cells.  |  Zhang, YP., et al. 2007. Exp Cell Res. 313: 3210-21. PMID: 17658515
8. The nuclear migration protein NUDF/LIS1 forms a complex with NUDC and BNFA at spindle pole bodies.  |  Helmstaedt, K., et al. 2008. Eukaryot Cell. 7: 1041-52. PMID: 18390647

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NUDC Antibody (C-3) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-NUDC Antibody (C-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NUDC Antibody (C-3) Specifications

Alternate Product Name: NUDC

Product Clone Name: C-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 61-331

Epitope Target: NUDC

Epitope Species: human

Product Reactivity/Detection: NUDC

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for NUDC Antibody (C-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin