Nucling Antibody (A-4): sc-514117
- Nucling Antibody (A-4) is a mouse monoclonal IgG1 κ Nucling antibody provided at 200 µg/ml
- raised against amino acids 301-600 mapping within an internal region of Nucling of human origin
- Nucling Antibody (A-4) is recommended for detection of Nucling of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Nucling Antibody (A-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Nucling Antibody (A-4) for WB applications. These reagents are now offered in bundles with Nucling Antibody (A-4) (see ordering information below).
QUICK LINKS
SEE ALSO...
Nucling Antibody (A-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Nucling protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Nucling antibody (A-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Nucling, also known as UACA (uveal autoantigen with coiled-coil domains and ankyrin repeats) and KIAA1561, is a 1,416 amino acid protein predominantly located in the nucleus and cytoplasm, where Nucling plays a crucial role in cellular apoptosis. Nucling functions as a key component of the apoptosome complex, which includes Apaf-1 and caspase-9, and is up-regulated following thyroid-stimulating hormone stimulation. Nucling directly interacts with Apaf-1, facilitating Apaf-1 redistribution to the nucleus in response to pro-apoptotic stress, thereby promoting apoptosis through pathways involving the down-regulation of galectin-3, up-regulation of the apoptosome, and inactivation of NFκB. Additionally, Nucling interacts with ARF6, potentially influencing cell shape and motility after injury. With high expression levels in the kidney, heart, pancreas, and skeletal muscle, Nucling is also recognized as a potential target autoantigen in conditions such as Behcet disease, Vogt-Koyanagi-Harada syndrome, and sarcoidosis, which are associated with various forms of pan-uveitis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Nucling Antibody (A-4) References:
- Identification of a novel autoantigen UACA in patients with panuveitis. | Yamada, K., et al. 2001. Biochem Biophys Res Commun. 280: 1169-76. PMID: 11162650
- A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway. | Bouwmeester, T., et al. 2004. Nat Cell Biol. 6: 97-105. PMID: 14743216
- Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation. | Brandenberger, R., et al. 2004. Nat Biotechnol. 22: 707-16. PMID: 15146197
- Large-scale characterization of HeLa cell nuclear phosphoproteins. | Beausoleil, SA., et al. 2004. Proc Natl Acad Sci U S A. 101: 12130-5. PMID: 15302935
- Detection of the novel autoantibody (anti-UACA antibody) in patients with Graves' disease. | Ohkura, T., et al. 2004. Biochem Biophys Res Commun. 321: 432-40. PMID: 15358194
- Coeliac disease-associated risk variants in TNFAIP3 and REL implicate altered NF-kappaB signalling. | Trynka, G., et al. 2009. Gut. 58: 1078-83. PMID: 19240061
- Lysine acetylation targets protein complexes and co-regulates major cellular functions. | Choudhary, C., et al. 2009. Science. 325: 834-40. PMID: 19608861
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nucling Antibody (A-4) | sc-514117 | 200 µg/ml | $322.00 | |||
Nucling Antibody (A-4): m-IgG Fc BP-HRP Bundle | sc-527854 | 200 µg Ab; 10 µg BP | $361.00 | |||
Nucling Antibody (A-4): m-IgG1 BP-HRP Bundle | sc-533227 | 200 µg Ab; 20 µg BP | $361.00 | |||
Nucling Antibody (A-4) AC | sc-514117 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Nucling Antibody (A-4) HRP | sc-514117 HRP | 200 µg/ml | $322.00 | |||
Nucling Antibody (A-4) FITC | sc-514117 FITC | 200 µg/ml | $336.00 | |||
Nucling Antibody (A-4) PE | sc-514117 PE | 200 µg/ml | $349.00 | |||
Nucling Antibody (A-4) Alexa Fluor® 488 | sc-514117 AF488 | 200 µg/ml | $364.00 | |||
Nucling Antibody (A-4) Alexa Fluor® 546 | sc-514117 AF546 | 200 µg/ml | $364.00 | |||
Nucling Antibody (A-4) Alexa Fluor® 594 | sc-514117 AF594 | 200 µg/ml | $364.00 | |||
Nucling Antibody (A-4) Alexa Fluor® 647 | sc-514117 AF647 | 200 µg/ml | $364.00 | |||
Nucling Antibody (A-4) Alexa Fluor® 680 | sc-514117 AF680 | 200 µg/ml | $364.00 | |||
Nucling Antibody (A-4) Alexa Fluor® 790 | sc-514117 AF790 | 200 µg/ml | $364.00 |