NTF2 Antibody (B-7): sc-271693
- NTF2 Antibody (B-7) is a mouse monoclonal IgG2b κ NTF2 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 97-127 at the C-terminus of nuclear transport factor 2 (NTF2) of human origin
- NTF2 Antibody (B-7) is recommended for detection of NTF2 p10 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-NTF2 Antibody (B-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for NTF2 Antibody (B-7) for WB applications. These reagents are now offered in bundles with NTF2 Antibody (B-7) (see ordering information below).
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NTF2 Antibody (B-7) is a mouse monoclonal IgG2b kappa light chain antibody that detects NTF2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NTF2 (B-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NTF2 plays a crucial role in nuclear transport, specifically in protein import into the nucleus. NTF2 functions as a homodimer that interacts with nucleoporins, such as p62, which are essential components of the nuclear pore complex. This interaction facilitates recognition and docking of import substrates at the nuclear pore, ensuring efficient protein transport into the nucleus. Proper functioning of NTF2 and associated proteins maintains cellular homeostasis and regulates gene expression, as disruption in nuclear transport can lead to various diseases, including cancer and neurodegenerative disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
NTF2 Antibody (B-7) References:
- Macromolecular interactions in the nucleoporin p62 complex of rat nuclear pores: binding of nucleoporin p54 to the rod domain of p62. | Buss, F. and Stewart, M. 1995. J Cell Biol. 128: 251-61. PMID: 7531196
- Direct interaction of nucleoporin p62 with mRNA during its export from the nucleus. | Dargemont, C., et al. 1995. J Cell Sci. 108 (Pt 1): 257-63. PMID: 7738103
- Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62. | Paschal, BM. and Gerace, L. 1995. J Cell Biol. 129: 925-37. PMID: 7744965
- Protein export from the nucleus requires the GTPase Ran and GTP hydrolysis. | Moroianu, J. and Blobel, G. 1995. Proc Natl Acad Sci U S A. 92: 4318-22. PMID: 7753805
- Previously identified protein of uncertain function is karyopherin alpha and together with karyopherin beta docks import substrate at nuclear pore complexes. | Moroianu, J., et al. 1995. Proc Natl Acad Sci U S A. 92: 2008-11. PMID: 7892216
- Ran binding domains promote the interaction of Ran with p97/beta-karyopherin, linking the docking and translocation steps of nuclear import. | Lounsbury, KM., et al. 1996. J Biol Chem. 271: 2357-60. PMID: 8576188
- The binding site of karyopherin alpha for karyopherin beta overlaps with a nuclear localization sequence. | Moroianu, J., et al. 1996. Proc Natl Acad Sci U S A. 93: 6572-6. PMID: 8692858
- Nuclear protein import: Ran-GTP dissociates the karyopherin alphabeta heterodimer by displacing alpha from an overlapping binding site on beta. | Moroianu, J., et al. 1996. Proc Natl Acad Sci U S A. 93: 7059-62. PMID: 8692944
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NTF2 Antibody (B-7) | sc-271693 | 200 µg/ml | $322.00 | |||
NTF2 Antibody (B-7): m-IgGκ BP-HRP Bundle | sc-522026 | 200 µg Ab, 40 µg BP | $361.00 | |||
NTF2 Antibody (B-7): m-IgG2b BP-HRP Bundle | sc-548993 | 200 µg Ab; 10 µg BP | $361.00 | |||
NTF2 Antibody (B-7) AC | sc-271693 AC | 500 µg/ml, 25% agarose | $424.00 | |||
NTF2 Antibody (B-7) HRP | sc-271693 HRP | 200 µg/ml | $322.00 | |||
NTF2 Antibody (B-7) FITC | sc-271693 FITC | 200 µg/ml | $336.00 | |||
NTF2 Antibody (B-7) PE | sc-271693 PE | 200 µg/ml | $349.00 | |||
NTF2 Antibody (B-7) Alexa Fluor® 488 | sc-271693 AF488 | 200 µg/ml | $364.00 | |||
NTF2 Antibody (B-7) Alexa Fluor® 546 | sc-271693 AF546 | 200 µg/ml | $364.00 | |||
NTF2 Antibody (B-7) Alexa Fluor® 594 | sc-271693 AF594 | 200 µg/ml | $364.00 | |||
NTF2 Antibody (B-7) Alexa Fluor® 647 | sc-271693 AF647 | 200 µg/ml | $364.00 | |||
NTF2 Antibody (B-7) Alexa Fluor® 680 | sc-271693 AF680 | 200 µg/ml | $364.00 | |||
NTF2 Antibody (B-7) Alexa Fluor® 790 | sc-271693 AF790 | 200 µg/ml | $364.00 | |||
NTF2 (B-7) Neutralizing Peptide | sc-271693 P | 100 µg/0.5 ml | $69.00 |