Date published: 2025-9-16

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NT5C3L Antibody (A-9): sc-398604

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Datasheets
  • NT5C3L Antibody (A-9) is a mouse monoclonal IgG1 κ NT5C3L antibody, cited in 1 publications, provided at 200 µg/ml
  • raised against amino acids 56-98 mapping near the N-terminus of NT5C3L of human origin
  • NT5C3L Antibody (A-9) is recommended for detection of NT5C3L of mouse, rat and human origin by WB, IP, IF and ELISA
  • Anti-NT5C3L Antibody (A-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of NT5C3L (A-9): sc-398604.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for NT5C3L Antibody (A-9) for WB applications. These reagents are now offered in bundles with NT5C3L Antibody (A-9) (see ordering information below).

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NT5C3L Antibody (A-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects NT5C3L protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NT5C3L (A-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NT5C3L, also known as 5′-nucleotidase, cytosolic III-like, is a 292 amino acid cytoplasmic protein that catalyzes the hydrolysis of nucleotides into nucleosides and phosphates. This enzymatic activity maintains nucleotide homeostasis within cells, supporting DNA and RNA synthesis, energy metabolism, and signal transduction. NT5C3L plays a key role in lymphocytes by helping regulate immune responses. As part of the pyrimidine 5′-nucleotidase family, NT5C3L functions as both a nucleotidase and phosphotransferase, highlighting its importance in cellular metabolism.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

NT5C3L Antibody (A-9) References:

  1. Human erythrocyte pyrimidine 5-nucleotidase, PN-I, is identical to p36, a protein associated to lupus inclusion formation in response to alpha-interferon.  |  Amici, A., et al. 2000. Blood. 96: 1596-8. PMID: 10942414
  2. Pyrimidine 5' nucleotidase deficiency.  |  Rees, DC., et al. 2003. Br J Haematol. 120: 375-83. PMID: 12580951
  3. Molecular characterization of Turkish patients with pyrimidine 5' nucleotidase-I deficiency.  |  Balta, G., et al. 2003. Blood. 102: 1900-3. PMID: 12714505
  4. Molecular characterization of six unrelated Italian patients affected by pyrimidine 5'-nucleotidase deficiency.  |  Bianchi, P., et al. 2003. Br J Haematol. 122: 847-51. PMID: 12930399
  5. Functional analysis of pyrimidine 5'-nucleotidase mutants causing nonspherocytic hemolytic anemia.  |  Chiarelli, LR., et al. 2005. Blood. 105: 3340-5. PMID: 15604219
  6. Overexpression of hUMP1/POMP proteasome accessory protein enhances proteasome-mediated antioxidant defence.  |  Chondrogianni, N. and Gonos, ES. 2007. Exp Gerontol. 42: 899-903. PMID: 17349762
  7. Homogeneous pyrimidine nucleotidase from human erythrocytes: enzymic and molecular properties.  |  Amici, A., et al. 1994. Biochem J. 304 (Pt 3): 987-92. PMID: 7818506

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

NT5C3L Antibody (A-9)

sc-398604
200 µg/ml
$316.00

NT5C3L Antibody (A-9): m-IgG Fc BP-HRP Bundle

sc-530867
200 µg Ab; 10 µg BP
$354.00

NT5C3L Antibody (A-9): m-IgGκ BP-HRP Bundle

sc-524416
200 µg Ab, 40 µg BP
$354.00

NT5C3L Antibody (A-9): m-IgG1 BP-HRP Bundle

sc-544289
200 µg Ab; 20 µg BP
$354.00

NT5C3L Antibody (A-9) AC

sc-398604 AC
500 µg/ml, 25% agarose
$416.00

NT5C3L Antibody (A-9) HRP

sc-398604 HRP
200 µg/ml
$316.00

NT5C3L Antibody (A-9) FITC

sc-398604 FITC
200 µg/ml
$330.00

NT5C3L Antibody (A-9) PE

sc-398604 PE
200 µg/ml
$343.00

NT5C3L Antibody (A-9) Alexa Fluor® 488

sc-398604 AF488
200 µg/ml
$357.00

NT5C3L Antibody (A-9) Alexa Fluor® 546

sc-398604 AF546
200 µg/ml
$357.00

NT5C3L Antibody (A-9) Alexa Fluor® 594

sc-398604 AF594
200 µg/ml
$357.00

NT5C3L Antibody (A-9) Alexa Fluor® 647

sc-398604 AF647
200 µg/ml
$357.00

NT5C3L Antibody (A-9) Alexa Fluor® 680

sc-398604 AF680
200 µg/ml
$357.00

NT5C3L Antibody (A-9) Alexa Fluor® 790

sc-398604 AF790
200 µg/ml
$357.00

What blocking solution should I use with NT5C3L (A-9): sc-398604 monoclonal antibody for Western blot?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Great Western Blot data of NT5C3L expressionGreat Western Blot data of NT5C3L expression in non-transfected and human NT5C3L transfected 293T whole cell lysates and human liver and human testis tissue extracts. -SCBT QC
Date published: 2015-01-04
Rated 5 out of 5 by from Good Western Blot data of NT5C3L expressionGood Western Blot data of NT5C3L expression in human stomach tissue extract and Hep G2 whole cell lysate . -SCBT QC
Date published: 2014-03-01
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NT5C3L Antibody (A-9) is rated 5.0 out of 5 by 2.
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