NSF Antibody (E-6): sc-74458

NSF Antibody (E-6) is a mouse monoclonal IgG1 antibody that detects NSF in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NSF, or N-ethylmaleimide-sensitive factor, plays a crucial role in the process of membrane fusion, which is essential for various cellular functions, including neurotransmitter release and vesicle trafficking. Anti-NSF antibody (E-6) targets a protein primarily located in the cytoplasm, where NSF interacts with soluble NSF attachment proteins (SNAPs) and syntaxins to facilitate the assembly of the SNARE complex, a key component in the exocytosis pathway. Proper functioning of NSF is vital for maintaining synaptic transmission and cellular communication, making NSF a significant target for research in neurobiology and cell biology. NSF monoclonal antibody (E-6) provides a reliable tool for studying the dynamics of membrane fusion and the molecular mechanisms underlying synaptic function, thereby contributing to our understanding of various physiological and pathological processes.

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NSF Antibody (E-6) References:

1. N-ethylmaleimide-sensitive factor is associated with the nuclear envelope.  |  Mashima, J., et al. 2000. Biochem Biophys Res Commun. 274: 559-64. PMID: 10913377
2. Unraveling the mechanism of the vesicle transport ATPase NSF, the N-ethylmaleimide-sensitive factor.  |  May, AP., et al. 2001. J Biol Chem. 276: 21991-4. PMID: 11301340
3. N-ethylmaleimide-sensitive factor is required to organize functional exocytotic microdomains in paramecium.  |  Froissard, M., et al. 2002. Genetics. 161: 643-50. PMID: 12072461
4. The activity of Golgi transport vesicles depends on the presence of the N-ethylmaleimide-sensitive factor (NSF) and a soluble NSF attachment protein (alpha SNAP) during vesicle formation.  |  Wattenberg, BW., et al. 1992. J Cell Biol. 118: 1321-32. PMID: 1522110
5. Thioredoxin increases exocytosis by denitrosylating N-ethylmaleimide-sensitive factor.  |  Ito, T., et al. 2011. J Biol Chem. 286: 11179-84. PMID: 21324905
6. Processive ATP-driven substrate disassembly by the N-ethylmaleimide-sensitive factor (NSF) molecular machine.  |  Cipriano, DJ., et al. 2013. J Biol Chem. 288: 23436-45. PMID: 23775070
7. Disassembly of all SNARE complexes by N-ethylmaleimide-sensitive factor (NSF) is initiated by a conserved 1:1 interaction between α-soluble NSF attachment protein (SNAP) and SNARE complex.  |  Vivona, S., et al. 2013. J Biol Chem. 288: 24984-91. PMID: 23836889
8. Three αSNAP and 10 ATP molecules are used in SNARE complex disassembly by N-ethylmaleimide-sensitive factor (NSF).  |  Shah, N., et al. 2015. J Biol Chem. 290: 2175-88. PMID: 25492864
9. Association of N-ethylmaleimide-sensitive factor with synaptic vesicles.  |  Hong, RM., et al. 1994. FEBS Lett. 350: 253-7. PMID: 8070574
10. Interaction of the N-ethylmaleimide-sensitive factor with AMPA receptors.  |  Song, I., et al. 1998. Neuron. 21: 393-400. PMID: 9728920

The preferred NSF antibody is NSF Antibody (A-3): sc-515043.