NSE1 Antibody (D-2): sc-376585

NSE1 Antibody (D-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects NSE1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NSE1 (D-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NSE1 plays a vital role in DNA repair, particularly in double-stranded breaks that can occur during DNA replication or due to exposure to damaging agents. Efficient repair of these breaks is essential for maintaining genomic stability and preventing cell death. NSE1 functions as a key component of the structural maintenance of chromosomes (SMC) complex, which includes SMC5 and SMC6, along with six conserved non-SMC subunits. This complex is crucial for sister chromatid homologous recombination DNA repair and helps prevent chromosomal rearrangements. A RING-like motif in NSE1 enhances DNA repair capabilities of the SMC5/SMC6 complex, and complete deletion of NSE1 is lethal to cells, underscoring its importance in cellular survival. Additionally, NSE2, another protein in this complex, facilitates the sumoylation of SMC6 and the DNA repair protein TRAX, and NSE2 depletion can lead to increased susceptibility to DNA damage-induced apoptosis.

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NSE1 Antibody (D-2) References:

1. The role of SMC proteins in the responses to DNA damage.  |  Lehmann, AR. 2005. DNA Repair (Amst). 4: 309-14. PMID: 15661654
2. SMC5 and SMC6 genes are required for the segregation of repetitive chromosome regions.  |  Torres-Rosell, J., et al. 2005. Nat Cell Biol. 7: 412-9. PMID: 15793567
3. The importance of being Smc5/6.  |  Watanabe, Y. 2005. Nat Cell Biol. 7: 329-31. PMID: 15803128
4. Human MMS21/NSE2 is a SUMO ligase required for DNA repair.  |  Potts, PR. and Yu, H. 2005. Mol Cell Biol. 25: 7021-32. PMID: 16055714
5. A new SUMO ligase in the DNA damage response.  |  Lee, KM. and O'Connell, MJ. 2006. DNA Repair (Amst). 5: 138-41. PMID: 16198156
6. Chromosomal association of the Smc5/6 complex reveals that it functions in differently regulated pathways.  |  Betts Lindroos, H., et al. 2006. Mol Cell. 22: 755-767. PMID: 16793545
7. Human SMC5/6 complex promotes sister chromatid homologous recombination by recruiting the SMC1/3 cohesin complex to double-strand breaks.  |  Potts, PR., et al. 2006. EMBO J. 25: 3377-88. PMID: 16810316
8. Smc5-Smc6 mediate DNA double-strand-break repair by promoting sister-chromatid recombination.  |  De Piccoli, G., et al. 2006. Nat Cell Biol. 8: 1032-4. PMID: 16892052
9. Nse1 RING-like domain supports functions of the Smc5-Smc6 holocomplex in genome stability.  |  Pebernard, S., et al. 2008. Mol Biol Cell. 19: 4099-109. PMID: 18667531
10. Structure Basis for Shaping the Nse4 Protein by the Nse1 and Nse3 Dimer within the Smc5/6 Complex.  |  Jo, A., et al. 2021. J Mol Biol. 433: 166910. PMID: 33676928
11. Role of Nse1 Subunit of SMC5/6 Complex as a Ubiquitin Ligase.  |  Kolesar, P., et al. 2022. Cells. 11: PMID: 35011726
12. Crucial role of the NSE1 RING domain in Smc5/6 stability and FANCM-independent fork progression.  |  Lorite, NP., et al. 2024. Cell Mol Life Sci. 81: 251. PMID: 38847937

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NSE1 Antibody (D-2) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-NSE1 Antibody (D-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NSE1 Antibody (D-2) Specifications

Alternate Product Name: NSE1

Product Clone Name: D-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-178

Epitope Target: NSE1

Epitope Species: human

Product Reactivity/Detection: NSE1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for NSE1 Antibody (D-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin