NRIP Antibody (B-11): sc-515639

NRIP Antibody (B-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects NRIP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NRIP (B-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NRIP protein, also known as IQWD1, MSTP055, ARCAP, or PC326, is an 860 amino acid protein that plays a crucial role as a ligand-dependent coactivator of nuclear receptors, enhancing transcriptional activity of androgen receptor and glucocorticoid receptor. NRIP protein is primarily localized in the nucleus and is expressed in tissues such as testis, skeletal muscle, prostate, and heart, where NRIP protein is involved in regulating gene expression in response to hormonal signals. NRIP protein contains one IQ domain and seven WD-repeats, which are structural motifs that contribute to its function in various cellular processes, including chromatin assembly, cell cycle control, and signal transduction. These WD-repeats facilitate protein-protein interactions, allowing NRIP protein to participate in complex regulatory networks essential for maintaining cellular homeostasis and responding to external stimuli. NRIP protein exists in three isoforms generated through alternative splicing, which may provide functional diversity and adaptability in different cellular contexts.

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NRIP Antibody (B-11) References:

1. Multiple androgen response elements and a Myc consensus site in the androgen receptor (AR) coding region are involved in androgen-mediated up-regulation of AR messenger RNA.  |  Grad, JM., et al. 1999. Mol Endocrinol. 13: 1896-911. PMID: 10551783
2. A novel tumour associated leucine zipper protein targeting to sites of gene transcription and splicing.  |  Türeci, O., et al. 2002. Oncogene. 21: 3879-88. PMID: 12032826
3. NRIP, a novel nuclear receptor interaction protein, enhances the transcriptional activity of nuclear receptors.  |  Tsai, TC., et al. 2005. J Biol Chem. 280: 20000-9. PMID: 15784617
4. Nuclear receptor interaction protein, a coactivator of androgen receptors (AR), is regulated by AR and Sp1 to feed forward and activate its own gene expression through AR protein stability.  |  Chen, PH., et al. 2008. Nucleic Acids Res. 36: 51-66. PMID: 17984071
5. Nuclear Receptor Interaction Protein (NRIP) expression assay using human tissue microarray and immunohistochemistry technology confirming nuclear localization.  |  Han, CP., et al. 2008. J Exp Clin Cancer Res. 27: 25. PMID: 18673574
6. NRIP, a novel calmodulin binding protein, activates calcineurin to dephosphorylate human papillomavirus E2 protein.  |  Chang, SW., et al. 2011. J Virol. 85: 6750-63. PMID: 21543494
7. nRIP-seq: a technique to identify RNA targets of an RNA binding protein on a genome-wide scale.  |  Zhao, JC. 2015. Methods Mol Biol. 1206: 97-106. PMID: 25240890
8. NRIP is newly identified as a Z-disc protein, activating calmodulin signaling for skeletal muscle contraction and regeneration.  |  Chen, HH., et al. 2015. J Cell Sci. 128: 4196-209. PMID: 26430214
9. NRIP/DCAF6 stabilizes the androgen receptor protein by displacing DDB2 from the CUL4A-DDB1 E3 ligase complex in prostate cancer.  |  Chen, HH., et al. 2017. Oncotarget. 8: 21501-21515. PMID: 28212551
10. Deficiency of nuclear receptor interaction protein leads to cardiomyopathy by disrupting sarcomere structure and mitochondrial respiration.  |  Yang, KC., et al. 2019. J Mol Cell Cardiol. 137: 9-24. PMID: 31629737
11. LC3B drives transcription-associated homologous recombination via direct interaction with R-loops.  |  Yoon, J., et al. 2024. Nucleic Acids Res. 52: 5088-5106. PMID: 38412240
12. The Innovative Role of Nuclear Receptor Interaction Protein in Orchestrating Invadosome Formation for Myoblast Fusion.  |  Chen, HH., et al. 2024. J Cachexia Sarcopenia Muscle. 15: 2559-2573. PMID: 39323088

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NRIP Antibody (B-11) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-NRIP Antibody (B-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NRIP Antibody (B-11) Specifications

Alternate Product Name: NRIP

Product Clone Name: B-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 711-880

Epitope Target: NRIP

Epitope Species: human

Product Reactivity/Detection: NRIP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for NRIP Antibody (B-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin