Nrf2 Antibody (A-10): sc-365949
- Nrf2 Antibody (A-10) is a mouse monoclonal IgG1 κ Nrf2 antibody, cited in 374 publications, provided at 200 µg/ml
- raised against amino acids 37-336 of Nrf2 of human origin
- Nrf2 Antibody (A-10) is recommended for detection of ~60 kDa forms of Nrf2 of human origin by WB, IP, IF and ELISA
- Anti-Nrf2 Antibody (A-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for Gel Supershift and ChIP applications (sc-365949 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Nrf2 Antibody (A-10) for WB applications. These reagents are now offered in bundles with Nrf2 Antibody (A-10) (see ordering information below).
Nrf2 Antibody (A-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects amino acids 37-336 of Nrf2 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Nrf2 monoclonal antibody (A-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Nrf2, also known as NF-E2-related factor 2, plays a crucial role in cellular defense mechanisms by regulating the expression of antioxidant and detoxifying enzymes, thereby protecting cells from oxidative stress and inflammation. Nrf2 is primarily located in the cytoplasm under normal conditions, where Keap1 maintains regulatory control. Upon exposure to oxidative stress or xenobiotics, Nrf2 translocates to the nucleus, where binding to antioxidant response elements (ARE) in promoter regions of target genes facilitates the transcription of protective proteins. This translocation enhances the expression of genes involved in detoxification and antioxidant defense, such as NAD(P)H quinone dehydrogenase 1 (NQO1). Anti-Nrf2 antibody (A-10) detects Nrf2 in both whole cell lysates and nuclear extracts, making this tool valuable for studying oxidative stress responses and related pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Nrf2 Antibody (A-10) References:
- Molecular cloning and functional characterization of a new Cap'n' collar family transcription factor Nrf3. | Kobayashi, A., et al. 1999. J Biol Chem. 274: 6443-52. PMID: 10037736
- Small maf (MafG and MafK) proteins negatively regulate antioxidant response element-mediated expression and antioxidant induction of the NAD(P)H:Quinone oxidoreductase1 gene. | Dhakshinamoorthy, S. and Jaiswal, AK. 2000. J Biol Chem. 275: 40134-41. PMID: 11013233
- Regulation of genes encoding NAD(P)H:quinone oxidoreductases. | Jaiswal, AK. 2000. Free Radic Biol Med. 29: 254-62. PMID: 11035254
- Impaired expression of glutathione synthetic enzyme genes in mice with targeted deletion of the Nrf2 basic-leucine zipper protein. | Chan, JY. and Kwong, M. 2000. Biochim Biophys Acta. 1517: 19-26. PMID: 11118612
- TCF11/Nrf1 overexpression increases the intracellular glutathione level and can transactivate the gamma-glutamylcysteine synthetase (GCS) heavy subunit promoter. | Myhrstad, MC., et al. 2001. Biochim Biophys Acta. 1517: 212-9. PMID: 11342101
- Nrf2-mediated protection against 6-hydroxydopamine. | Jakel, RJ., et al. 2007. Brain Res. 1144: 192-201. PMID: 17336276
- Functional polymorphisms in the transcription factor NRF2 in humans increase the risk of acute lung injury. | Marzec, JM., et al. 2007. FASEB J. 21: 2237-46. PMID: 17384144
- Chromosomal localization of the human NF-E2 family of bZIP transcription factors by fluorescence in situ hybridization. | Chan, JY., et al. 1995. Hum Genet. 95: 265-9. PMID: 7868116
- NRF2, a member of the NFE2 family of transcription factors, is not essential for murine erythropoiesis, growth, and development. | Chan, K., et al. 1996. Proc Natl Acad Sci U S A. 93: 13943-8. PMID: 8943040
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nrf2 Antibody (A-10) | sc-365949 | 200 µg/ml | $316.00 | |||
Nrf2 Antibody (A-10): m-IgG Fc BP-HRP Bundle | sc-537857 | 200 µg Ab; 10 µg BP | $354.00 | |||
Nrf2 Antibody (A-10): m-IgGκ BP-HRP Bundle | sc-535285 | 200 µg Ab; 40 µg BP | $354.00 | |||
Nrf2 Antibody (A-10): m-IgG1 BP-HRP Bundle | sc-545408 | 200 µg Ab; 20 µg BP | $354.00 | |||
Nrf2 Antibody (A-10) X | sc-365949 X | 200 µg/0.1 ml | $316.00 | |||
Nrf2 Antibody (A-10) AC | sc-365949 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Nrf2 Antibody (A-10) HRP | sc-365949 HRP | 200 µg/ml | $316.00 | |||
Nrf2 Antibody (A-10) FITC | sc-365949 FITC | 200 µg/ml | $330.00 | |||
Nrf2 Antibody (A-10) PE | sc-365949 PE | 200 µg/ml | $343.00 | |||
Nrf2 Antibody (A-10) Alexa Fluor® 488 | sc-365949 AF488 | 200 µg/ml | $357.00 | |||
Nrf2 Antibody (A-10) Alexa Fluor® 546 | sc-365949 AF546 | 200 µg/ml | $357.00 | |||
Nrf2 Antibody (A-10) Alexa Fluor® 594 | sc-365949 AF594 | 200 µg/ml | $357.00 | |||
Nrf2 Antibody (A-10) Alexa Fluor® 647 | sc-365949 AF647 | 200 µg/ml | $357.00 | |||
Nrf2 Antibody (A-10) Alexa Fluor® 680 | sc-365949 AF680 | 200 µg/ml | $357.00 | |||
Nrf2 Antibody (A-10) Alexa Fluor® 790 | sc-365949 AF790 | 200 µg/ml | $357.00 |