Nrf1 Antibody (H-4): sc-28379

Nrf1 Antibody (H-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects Nrf1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Nrf1 antibody (H-4) is raised against amino acids 191-475 of Nrf1 of human origin. Nrf1 (H-4) antibody is available as the non-conjugated form. Nrf1, also known as nuclear factor erythroid 2-related factor 1, plays a crucial role in cellular defense mechanisms by regulating the expression of genes involved in the antioxidant response and detoxification processes. Nrf1 belongs to the Cap ′n′ collar (CNC) family of transcription factors, which includes Nrf2 and Nrf3, and is characterized by its basic leucine zipper (bZIP) domain that facilitates dimerization with Maf proteins to bind to specific DNA sequences known as Maf recognition elements (MAREs). Nrf1′s ability to heterodimerize with Jun family members, such as c-Jun, JunB, and JunD, enhances its capacity to activate the expression of detoxifying enzymes, including NAD(P)H quinone dehydrogenase 1 (NQO1), thereby contributing to the cellular response to oxidative stress. Understanding Nrf1′s function is vital, as Nrf1 not only helps in maintaining cellular homeostasis but also plays a significant role in protecting against various diseases linked to oxidative damage, making Nrf1 a key target for therapeutic interventions.

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Nrf1 Antibody (H-4) References:

1. Molecular cloning and functional characterization of a new Cap'n' collar family transcription factor Nrf3.  |  Kobayashi, A., et al. 1999. J Biol Chem. 274: 6443-52. PMID: 10037736
2. Small maf (MafG and MafK) proteins negatively regulate antioxidant response element-mediated expression and antioxidant induction of the NAD(P)H:Quinone oxidoreductase1 gene.  |  Dhakshinamoorthy, S. and Jaiswal, AK. 2000. J Biol Chem. 275: 40134-41. PMID: 11013233
3. Regulation of genes encoding NAD(P)H:quinone oxidoreductases.  |  Jaiswal, AK. 2000. Free Radic Biol Med. 29: 254-62. PMID: 11035254
4. Impaired expression of glutathione synthetic enzyme genes in mice with targeted deletion of the Nrf2 basic-leucine zipper protein.  |  Chan, JY. and Kwong, M. 2000. Biochim Biophys Acta. 1517: 19-26. PMID: 11118612
5. TCF11/Nrf1 overexpression increases the intracellular glutathione level and can transactivate the gamma-glutamylcysteine synthetase (GCS) heavy subunit promoter.  |  Myhrstad, MC., et al. 2001. Biochim Biophys Acta. 1517: 212-9. PMID: 11342101
6. Negative regulation of the Nrf1 transcription factor by its N-terminal domain is independent of Keap1: Nrf1, but not Nrf2, is targeted to the endoplasmic reticulum.  |  Zhang, Y., et al. 2006. Biochem J. 399: 373-85. PMID: 16872277
7. Orphan nuclear receptor estrogen-related receptor alpha is essential for adaptive thermogenesis.  |  Villena, JA., et al. 2007. Proc Natl Acad Sci U S A. 104: 1418-23. PMID: 17229846
8. Chromosomal localization of the human NF-E2 family of bZIP transcription factors by fluorescence in situ hybridization.  |  Chan, JY., et al. 1995. Hum Genet. 95: 265-9. PMID: 7868116
9. NRF2, a member of the NFE2 family of transcription factors, is not essential for murine erythropoiesis, growth, and development.  |  Chan, K., et al. 1996. Proc Natl Acad Sci U S A. 93: 13943-8. PMID: 8943040

The preferred Nrf1 antibody is Nrf1 Antibody (G-5): sc-515360.