NRBF2 Antibody (D-3): sc-365213
- NRBF2 Antibody (D-3) is a mouse monoclonal IgG1 κ NRBF2 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-93 mapping at the N-terminus of NRBF2 of human origin
- NRBF2 Antibody (D-3) is recommended for detection of NRBF2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-NRBF2 Antibody (D-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP is the preferred secondary detection reagent for NRBF2 Antibody (D-3) for WB and IHC(P) applications. This reagent is now offered in a bundle with NRBF2 Antibody (D-3) (see ordering information below).
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SEE ALSO...
NRBF2 Antibody (D-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects NRBF2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-NRBF2 antibody (D-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NRBF2, also known as Nuclear receptor-binding factor 2 or Comodulator of PPAR and RXR, plays a crucial role as a transcriptional coactivator, significantly influencing nuclear hormone receptor activity. These receptors, when bound to specific ligands, undergo conformational changes that enable recruitment of transcriptional coactivators like NRBF2, thereby enhancing gene expression. NRBF2 is predominantly expressed in liver, placenta, and keratinocytes, and interacts with at least seven nuclear receptors, including PPARα, PPARδ, and PPARγ, facilitating transcriptional activity. NRBF2 can also bind to retinoic acid receptors RARα and RARγ, as well as retinoid X receptor RXRα, in the presence of ligands. NRBF2 exists in two isoforms due to alternative splicing and is localized in both nucleus and cytoplasm, essential for regulating gene expression in response to hormonal signals.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
NRBF2 Antibody (D-3) References:
- Nuclear receptor binding factor-2 (NRBF-2), a possible gene activator protein interacting with nuclear hormone receptors. | Yasumo, H., et al. 2000. Biochim Biophys Acta. 1490: 189-97. PMID: 10786636
- FHL2 expression in keratinocytes and transcriptional effect on PPARalpha/RXRalpha. | Ciarlo, JD., et al. 2004. J Dermatol Sci. 35: 61-3. PMID: 15194148
- Isolation and functional analysis of a keratinocyte-derived, ligand-regulated nuclear receptor comodulator. | Flores, AM., et al. 2004. J Invest Dermatol. 123: 1092-101. PMID: 15610520
- Enzyme association with PPARgamma: evidence of a new role for 15-lipoxygenase type 2. | Flores, AM., et al. 2005. Chem Biol Interact. 151: 121-32. PMID: 15698583
- A signature motif in transcriptional co-activators mediates binding to nuclear receptors. | Heery, DM., et al. 1997. Nature. 387: 733-6. PMID: 9192902
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NRBF2 Antibody (D-3) | sc-365213 | 200 µg/ml | $322.00 | |||
NRBF2 Antibody (D-3): m-IgG1 BP-HRP Bundle | sc-531703 | 200 µg Ab; 20 µg BP | $361.00 | |||
NRBF2 Antibody (D-3) AC | sc-365213 AC | 500 µg/ml, 25% agarose | $424.00 | |||
NRBF2 Antibody (D-3) HRP | sc-365213 HRP | 200 µg/ml | $322.00 | |||
NRBF2 Antibody (D-3) FITC | sc-365213 FITC | 200 µg/ml | $336.00 | |||
NRBF2 Antibody (D-3) PE | sc-365213 PE | 200 µg/ml | $349.00 | |||
NRBF2 Antibody (D-3) Alexa Fluor® 488 | sc-365213 AF488 | 200 µg/ml | $364.00 | |||
NRBF2 Antibody (D-3) Alexa Fluor® 546 | sc-365213 AF546 | 200 µg/ml | $364.00 | |||
NRBF2 Antibody (D-3) Alexa Fluor® 594 | sc-365213 AF594 | 200 µg/ml | $364.00 | |||
NRBF2 Antibody (D-3) Alexa Fluor® 647 | sc-365213 AF647 | 200 µg/ml | $364.00 | |||
NRBF2 Antibody (D-3) Alexa Fluor® 680 | sc-365213 AF680 | 200 µg/ml | $364.00 | |||
NRBF2 Antibody (D-3) Alexa Fluor® 790 | sc-365213 AF790 | 200 µg/ml | $364.00 |