NRBF2 Inhibitors
Chemical inhibitors of NRBF2 function by disrupting various stages of the autophagic process, where NRBF2 is known to play a crucial role. LY294002 and Wortmannin are both inhibitors of PI3K, an enzyme that is pivotal for the initiation of autophagy. By inhibiting PI3K, these chemicals prevent the formation of the autophagic vesicle, thereby obstructing the functional role of NRBF2 in autophagosome formation. Similarly, 3-Methyladenine targets the same initial stage of autophagy by inhibiting PI3K, resulting in a reduction of autophagic vesicles and indirectly inhibiting the activity of NRBF2. Spautin-1 operates downstream by targeting ubiquitin-specific peptidases USP10 and USP13, influencing the degradation process of autophagy and thus reducing the recycling of cellular components where NRBF2 is involved. SAR405 and Vps34-IN1 both inhibit Vps34 PI3 kinase, which is necessary for the nucleation of autophagic vesicles, leading to a decrease in autophagic activity and, consequently, NRBF2 inhibition.
Moreover, SBI-0206965 and Autophinib focus on other critical autophagic proteins, ULK1 and ATG4, respectively. SBI-0206965's inhibition of ULK1 disrupts the signaling required for autophagy induction, while Autophinib impairs ATG4's ability to process LC3-ATG8, an essential step in autophagosome maturation, thus inhibiting NRBF2. Bafilomycin A1 and Chloroquine both inhibit the fusion between autophagosomes and lysosomes by disrupting lysosomal acidification. This action prevents the final degradative steps of autophagy, thereby inhibiting NRBF2, which is implicated in the autophagic flux. NSC185058 disrupts ATG4B activity and impedes LC3-ATG8 processing, similar to Autophinib, which hinders autophagosome formation and function, leading to an indirect inhibition of NRBF2. Lastly, Lys05, as a more potent analog of Chloroquine, impedes lysosomal degradation, a stage critical for the completion of the autophagic cycle, thus functionally inhibiting NRBF2 by obstructing the autophagic flux.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
LY 294002 | 154447-36-6 | sc-201426 sc-201426A | 5 mg 25 mg | $123.00 $400.00 | 148 | |
LY294002 is a potent inhibitor of phosphatidylinositol 3-kinase (PI3K), which is upstream of the autophagic pathway where NRBF2 plays a critical role. Inhibition of PI3K leads to reduced autophagy, thereby inhibiting NRBF2 function. | ||||||
Autophagy Inhibitor, 3-MA | 5142-23-4 | sc-205596 sc-205596A | 50 mg 500 mg | $65.00 $261.00 | 113 | |
3-Methyladenine is a known inhibitor of PI3K, specifically at the initiation stage of autophagy, which is essential for NRBF2 function. Its action directly impedes autophagy, thereby inhibiting NRBF2. | ||||||
Wortmannin | 19545-26-7 | sc-3505 sc-3505A sc-3505B | 1 mg 5 mg 20 mg | $67.00 $223.00 $425.00 | 97 | |
Wortmannin is another PI3K inhibitor that prevents the initiation of autophagy. By blocking PI3K, it indirectly inhibits the functioning of NRBF2, which is involved in autophagic processes. | ||||||
Spautin-1 | 1262888-28-7 | sc-507306 | 10 mg | $168.00 | ||
Spautin-1 selectively inhibits the ubiquitin-specific peptidases USP10 and USP13 which are involved in the regulation of autophagy. Inhibiting these peptidases can lead to a reduction in autophagic flux, thus inhibiting NRBF2. | ||||||
SAR405 | 1523406-39-4 | sc-507416 | 1 mg | $125.00 | ||
SAR405 is a potent inhibitor of the Vps34 PI3 kinase complex, which is involved in the regulation of autophagy. Inhibition of Vps34 can lead to reduced autophagic activity, thereby functionally inhibiting NRBF2. | ||||||
SBI-0206965 | 1884220-36-3 | sc-507431 | 10 mg | $124.00 | ||
SBI-0206965 is a selective inhibitor of the kinase ULK1, which plays a pivotal role in the induction of autophagy. By inhibiting ULK1, it indirectly inhibits NRBF2 by impeding the autophagic process. | ||||||
Bafilomycin A1 | 88899-55-2 | sc-201550 sc-201550A sc-201550B sc-201550C | 100 µg 1 mg 5 mg 10 mg | $98.00 $255.00 $765.00 $1457.00 | 280 | |
Bafilomycin A1 is a specific inhibitor of vacuolar-type H+-ATPase (V-ATPase). By preventing the acidification of autophagosomes, it inhibits autophagic degradation, which is essential for NRBF2 function. | ||||||
Chloroquine | 54-05-7 | sc-507304 | 250 mg | $69.00 | 2 | |
Chloroquine, traditionally known as an antimalarial drug, inhibits autophagy by preventing lysosomal acidification, thus interfering with autophagosome clearance, which indirectly inhibits NRBF2 function. | ||||||
NSC 185058 | 39122-38-8 | sc-507531 | 1 mg | $85.00 | ||
NSC185058 disrupts ATG4B activity, an enzyme essential for autophagy. It inhibits the delipidation of LC3-ATG8, a step necessary for autophagosome formation, thus indirectly inhibiting NRBF2 which is associated with autophagy. | ||||||
Lys05 | 1391426-24-6 | sc-507532 | 5 mg | $140.00 | ||
Lys05 is a dimeric chloroquine which functions as an autophagy inhibitor more potent than chloroquine. It inhibits lysosomal autophagy, thereby functionally inhibiting NRBF2 by hindering the autophagic flux. | ||||||