Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
NRAGE Antibody (AK47) is a high quality monoclonal NRAGE antibody (also designated NRAGE antibody) suitable for the detection of the NRAGE protein of human origin. NRAGE Antibody (AK47) is available as the non-conjugated anti-NRAGE antibody. The neurotrophin family of growth factors (NGF) function to regulate neuronal differentiation, synaptic activity and neuronal survival, as well as axonal and dendritic growth. The melanoma-associated antigen (MAGE) family consists of a number of antigens recognized by cytotoxic T lymphocytes. Neurotrophin receptor-interacting MAGE homolog (NRAGE) binds the p75 neurotrophin re-ceptor and associates with the plasma membrane when NGF binds p75NTR. The critical factors for NRAGE association lie within the juxtamembrane domain of p75NTR. Overexpression of NRAGE stimulates cell cycle arrest and allows NGF-dependent apoptosis within sympathetic neuron precursors cells. NRAGE is expressed in the medulla oblongata during development and motor-neurons. Structural similarities suggest NRAGE, and the MAGE protein necdin, (Ndn), mediate cell cycle effects through a shared mechanism.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
I am using NRAGE (AK47): sc-130434 antibody for immunoprecipitation, how much antibody should I use?
Asked by: Trav11
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here:
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn