NQO1 Antibody (H-9): sc-376023

NQO1 Antibody (H-9) is a mouse monoclonal IgG2b kappa light chain antibody that detects NQO1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). NQO1 (H-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NQO1, consisting of 274 amino acids, plays a crucial role in cellular defense by catalyzing the reduction of quinones to hydroquinones, thereby detoxifying harmful compounds and protecting cells from oxidative stress, cytotoxicity, and mutagenicity. This function is particularly important in cancer, as many tumors exhibit overexpression of NQO1, which serves as an obligate two-electron reductase that not only deactivates toxins but also activates bioreductive anticancer drugs. NQO1 gene expression is tightly regulated and can be induced by various stimuli, including xenobiotics, antioxidants, heavy metals, and radiation, with the antioxidant response element (ARE) in the promoter region being essential for this regulation. Key transcription factors such as Nrf2, c-Jun, and Maf proteins interact with the ARE to modulate NQO1 expression, highlighting the intricate network of cellular responses that govern NQO1′s activity and implications in cancer biology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NQO1 Antibody (H-9) has 54 citations in a variety of scientific publications.

Primary antibodies like Anti-NQO1 Antibody (H-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NQO1 Antibody (H-9) Specifications

Alternate Product Name: NQO1

Product Clone Name: H-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 201-239

Epitope Target: NQO1

Epitope Species: human

Product Reactivity/Detection: NQO1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 54 citations in various scientific publications

Available Conjugates/Forms for NQO1 Antibody (H-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred NQO1 antibody is NQO1 Antibody (A180): sc-32793.