Notch 2 Antibody (F-10): sc-518169
- Notch 2 Antibody (F-10) is a mouse monoclonal IgM κ, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 248-272 of Notch 2 of human origin
- recommended for detection of Notch 2 of human origin by WB, IP, IF and ELISA
- Anti-Notch 2 Antibody (F-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for Notch 2 Antibody (F-10) for WB applications. This reagent is now offered in a bundle with Notch 2 Antibody (F-10) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Notch 2 (F-10) is a mouse monoclonal IgM kappa antibody that detects Notch 2 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF) and enzyme-linked immunosorbent assay (ELISA). Anti-Notch 2 antibody (F-10) is available as non-conjugated form, as well as multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC) and multiple Alexa Fluor® conjugates. The LIN-12/Notch family of transmembrane receptors plays a central role in development by regulating cell fate decisions. Four Notch homologs have been identified in mammals: Notch 1, Notch 2, Notch 3 and Notch 4. Notch genes are expressed in various tissues during embryonic and adult stages, suggesting involvement in multiple signaling pathways. Notch proteins have been found to be overexpressed or rearranged in human tumors. Ligands for Notch include Jagged, Jagged2 and Delta. Jagged can activate Notch and prevent myoblast differentiation by inhibiting the expression of muscle regulatory and structural genes. Jagged2 is involved in the development of various tissues dependent upon epithelial-mesenchymal interactions. Normal Delta expression is restricted to the adrenal gland and placenta. Delta expression has also been found in neuroendocrine tumors such as neuroblastomas and pheochromocytomas.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Notch 2 Antibody (F-10) References:
- Perspectives on the discovery of NOTCH2-specific inhibitors. | Dobranowski, P., et al. 2018. Chem Biol Drug Des. 91: 691-706. PMID: 29078041
- The endothelial Dll4-muscular Notch2 axis regulates skeletal muscle mass. | Fujimaki, S., et al. 2022. Nat Metab. 4: 180-189. PMID: 35228746
- DLBCL-associated NOTCH2 mutations escape ubiquitin-dependent degradation and promote chemoresistance. | Zhou, N., et al. 2023. Blood. 142: 973-988. PMID: 37235754
- NOTCH2 sensitizes the chondrocyte to the inflammatory response of tumor necrosis factor α. | Canalis, E., et al. 2023. J Biol Chem. 299: 105372. PMID: 37865314
- Pan-cancer tRNA-derived fragment CAT1 coordinates RBPMS to stabilize NOTCH2 mRNA to promote tumorigenesis. | Yu, M., et al. 2023. Cell Rep. 42: 113408. PMID: 37943661
- NOTCH2 promotes osteoclast maturation and metabolism and modulates the transcriptome profile during osteoclastogenesis. | Canalis, E., et al. 2024. J Biol Chem. 300: 105613. PMID: 38159855
- Notch2 controls developmental fate choices between germinal center and marginal zone B cells upon immunization. | Babushku, T., et al. 2024. Nat Commun. 15: 1960. PMID: 38438375
- LRP1 induces anti-PD-1 resistance by modulating the DLL4-NOTCH2-CCL2 axis and redirecting M2-like macrophage polarisation in bladder cancer. | Lin, H., et al. 2024. Cancer Lett. 593: 216807. PMID: 38462037
- Neutrophil extracellular traps promote immune escape in hepatocellular carcinoma by up-regulating CD73 through Notch2. | Yu, Y., et al. 2024. Cancer Lett. 598: 217098. PMID: 38969159
- Induction of a distinct macrophage population and protection from lung injury and fibrosis by Notch2 blockade. | Cruz Tleugabulova, M., et al. 2024. Nat Commun. 15: 9575. PMID: 39505846
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Notch 2 Antibody (F-10) | sc-518169 | 200 µg/ml | $316.00 | |||
Notch 2 Antibody (F-10): m-IgGκ BP-HRP Bundle | sc-550685 | 200 µg Ab; 40 µg BP | $354.00 | |||
Notch 2 Antibody (F-10) AC | sc-518169 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Notch 2 Antibody (F-10) HRP | sc-518169 HRP | 200 µg/ml | $316.00 | |||
Notch 2 Antibody (F-10) FITC | sc-518169 FITC | 200 µg/ml | $330.00 | |||
Notch 2 Antibody (F-10) PE | sc-518169 PE | 200 µg/ml | $343.00 | |||
Notch 2 Antibody (F-10) Alexa Fluor® 488 | sc-518169 AF488 | 200 µg/ml | $357.00 | |||
Notch 2 Antibody (F-10) Alexa Fluor® 546 | sc-518169 AF546 | 200 µg/ml | $357.00 | |||
Notch 2 Antibody (F-10) Alexa Fluor® 594 | sc-518169 AF594 | 200 µg/ml | $357.00 | |||
Notch 2 Antibody (F-10) Alexa Fluor® 647 | sc-518169 AF647 | 200 µg/ml | $357.00 | |||
Notch 2 Antibody (F-10) Alexa Fluor® 680 | sc-518169 AF680 | 200 µg/ml | $357.00 | |||
Notch 2 Antibody (F-10) Alexa Fluor® 790 | sc-518169 AF790 | 200 µg/ml | $357.00 |