Notch1 Antibody (A-8): sc-376403

Notch 1 Antibody (A-8) is a mouse monoclonal IgM kappa light chain antibody specifically engineered to detect Notch1, also known as Neurogenic locus notch homolog protein 1, in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA) applications. Notch1 monoclonal antibody (A-8) targets an epitope spanning amino acids 2425-2444 near the C-terminus of the Notch1 protein, ensuring precise recognition of the Notch1 precursor, mature Notch1, Notch1 NEXT, and Notch1 NICD forms across multiple species, including equine. Notch1 is a transmembrane receptor that plays a pivotal role in the Notch signaling pathway, which is essential for regulating cell fate decisions, differentiation, proliferation, and apoptosis during embryonic development and maintaining tissue homeostasis in adults. By interacting with ligands such as Jagged1, Jagged2, and Delta-like proteins, Notch1 facilitates critical communication between neighboring cells, influencing various developmental processes and ensuring proper cellular function. These interactions are vital for processes like vascular development, neural differentiation, and immune system regulation. Dysregulation of Notch1 signaling has been implicated in numerous diseases, including various cancers and developmental disorders, highlighting the importance in maintaining cellular balance and preventing pathological conditions. anti-Notch1 antibody (A-8) is available in both non-conjugated form and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, catering to diverse experimental needs.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Notch1 Antibody (A-8) has 50 citations in a variety of scientific publications.

Primary antibodies like Anti-Notch1 Antibody (A-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Notch1 Antibody (A-8) Specifications

Alternate Product Name: Notch 1

Product Clone Name: A-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 2425-2444

Epitope Target: Notch 1

Epitope Species: human

Product Reactivity/Detection: Notch 1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 50 citations in various scientific publications

Available Conjugates/Forms for Notch1 Antibody (A-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Notch1 antibody is Notch1 Antibody (mN1A): sc-32745.