NOS1 Antibody (G-4): sc-398843

NOS1 Antibody (G-4) is a mouse monoclonal IgG1 kappa light chain antibody developed to detect neuronal nitric oxide synthase (nNOS), also known as NOS1 or ncNOS. NOS1 monoclonal antibody (G-4) targets an epitope mapping between amino acids 37-56 near the N-terminus of the human NOS1 protein, ensuring effective recognition in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Neuronal nitric oxide synthase is an essential enzyme responsible for the production of nitric oxide (NO), a pivotal signaling molecule involved in neurotransmission, synaptic plasticity, and the regulation of neuronal communication. NOS1 is predominantly localized in neurons, where NOS1 interacts with key proteins such as PSD-95, which anchors NOS1 to the postsynaptic density, and calmodulin, which regulates enzymatic activity. These interactions are crucial for the precise spatial and temporal production of NO, facilitating NO′s role in modulating synaptic strength and plasticity. Understanding the protein-protein interactions of NOS1 is vital for elucidating the mechanisms underlying neuronal signaling and the pathophysiology of neurological disorders where NO signaling may be disrupted. Anti-NOS1 antibody (G-4) is available in both non-conjugated form and a variety of conjugated forms, including horseradish peroxidase (HRP), fluorescein isothiocyanate (FITC), and Alexa Fluor® conjugates, providing versatility for diverse experimental setups. By enabling the detailed study of NOS1 and its interacting partners, NOS1 (G-4) antibody supports advanced research into NO-mediated neuronal functions and their implications in health and disease, ultimately contributing to the development of targeted therapeutic strategies.

The preferred NOS1 antibody is NOS1 Antibody (A-11): sc-5302.