NOP2 Antibody (E-7): sc-398884

NOP2 Antibody (E-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects NOP2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NOP2 (E-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NOP2, also known as NOL1, p120, NOP120, or nucleolar protein 1, is an 812 amino acid nucleolar protein that plays a crucial role in ribosome assembly and the structural integrity of the nucleolus. NOP2 is classified within the methyltransferase superfamily and is primarily expressed during the G1 phase of the cell cycle, peaking in the early S phase. NOP2 functions as a ribosomal RNA methyltransferase, which is essential for the proper processing and maturation of rRNA, thereby influencing ribosome biogenesis and cellular proliferation. This function is particularly important as NOP2 links ribosome production to cell growth and division, making NOP2 a potential marker for proliferation in neoplastic cells and various cancer types. Additionally, the presence of two isoforms resulting from alternative splicing events adds complexity to NOP2′s regulatory roles in cellular processes.

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NOP2 Antibody (E-7) References:

1. Aurora-B regulates RNA methyltransferase NSUN2.  |  Sakita-Suto, S., et al. 2007. Mol Biol Cell. 18: 1107-17. PMID: 17215513
2. Nucleolar protein 1 (Nol1) expression in the mouse brain.  |  Mitrecić, D., et al. 2008. Coll Antropol. 32 Suppl 1: 123-6. PMID: 18405070
3. Phylogenetic analysis of the eukaryotic RNA (cytosine-5)-methyltransferases.  |  Pavlopoulou, A. and Kossida, S. 2009. Genomics. 93: 350-7. PMID: 19135144
4. Isolation and sequencing of NOP1. A yeast gene encoding a nucleolar protein homologous to a human autoimmune antigen.  |  Henríquez, R., et al. 1990. J Biol Chem. 265: 2209-15. PMID: 2298745
5. Human NOP2/NSUN1 regulates ribosome biogenesis through non-catalytic complex formation with box C/D snoRNPs.  |  Liao, H., et al. 2022. Nucleic Acids Res. 50: 10695-10716. PMID: 36161484
6. NOP2-mediated m5C Modification of c-Myc in an EIF3A-Dependent Manner to Reprogram Glucose Metabolism and Promote Hepatocellular Carcinoma Progression.  |  Zhang, H., et al. 2023. Research (Wash D C). 6: 0184. PMID: 37398932
7. NOP2-mediated m5C methylation of XPD is associated with hepatocellular carcinoma progression.  |  Sun, GF. and Ding, H. 2023. Neoplasma. 70: 340-349. PMID: 37498063
8. N4-acetylcytidine of Nop2 mRNA is required for the transition of morula-to-blastocyst.  |  Wang, M., et al. 2023. Cell Mol Life Sci. 80: 307. PMID: 37768430
9. NOP2 facilitates EZH2-mediated epithelial-mesenchymal transition by enhancing EZH2 mRNA stability via m5C methylation in lung cancer progression.  |  Yang, Y., et al. 2024. Cell Death Dis. 15: 506. PMID: 39013911
10. In vitro and ex vivo expression of nucleolar proteins B23 and p120 in benign and malignant epithelial lesions of the prostate.  |  Bocker, T., et al. 1995. Mod Pathol. 8: 226-31. PMID: 7542384
11. Assignment of the gene for the human proliferating cell nucleolar protein P120 (NOL1) to chromosome 12p13 by fluorescence in situ hybridization and polymerase chain reaction with somatic cell hybrids.  |  Baens, M., et al. 1994. Genomics. 21: 296-7. PMID: 8088812
12. Expression of nucleolar protein p120 in human lung cancer: difference in histological types as a marker for proliferation.  |  Uchiyama, B., et al. 1997. Clin Cancer Res. 3: 1873-7. PMID: 9815576

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NOP2 Antibody (E-7) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-NOP2 Antibody (E-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NOP2 Antibody (E-7) Specifications

Alternate Product Name: NOP2

Product Clone Name: E-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 241-389

Epitope Target: NOP2

Epitope Species: human

Product Reactivity/Detection: NOP2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for NOP2 Antibody (E-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent