Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Nop14 CRISPR/Cas9 KO Plasmid (h): sc-408567
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nop14 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Nop14 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Nop14 Antibody (B-5): sc-398763
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nop14 CRISPR/Cas9 KO Plasmid (h) | sc-408567 | 20 µg | $397.00 |
Overview
NOP14 encodes Nop14, a nucleolar protein required for ribosome biogenesis and maturation of the 18S rRNA through assembly and processing of the small ribosomal subunit. It participates in preribosomal particle formation and supports nucleolar organization, linking rRNA processing to cell growth control and proteostasis. Disruption of NOP14 can perturb translation capacity and nucleolar stress responses, processes that intersect with cell-cycle regulation and p53-associated checkpoints. Altered ribosome biogenesis programs involving NOP14 have been investigated in proliferative disorders and cancer biology as part of broader dysregulation of RNA processing and growth signaling.
Nop14 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NOP14 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NOP14 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NOP14 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Nop14 protein expression.
This CRISPR knockout system enables efficient generation of NOP14-deficient cell models for investigation of Nop14 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NOP14 exon(s) critical for Nop14 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NOP14 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Nop14 CRISPR/Cas9 KO Plasmid (h) and Nop14 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NOP14 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Nop14 HDR Plasmid (h) and Nop14 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NOP14 homology arms to support homology-directed repair at defined NOP14 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.