Nop14 Antibody (B-5): sc-398763

Nop14 Antibody (B-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects Nop14 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Nop14 antibody (B-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Nop14, also known as nucleolar protein 14 or NOL14, is a crucial 857 amino acid nuclear protein that plays a significant role in the processing of pre-18S ribosomal RNA within the nucleolus, which is essential for the formation of ribosomes, the cellular machinery responsible for protein synthesis. Nop14 exists in two alternatively spliced isoforms and is a key component of the ribosomal small subunit processome, facilitating the nuclear export of the 40S pre-ribosomal subunit to the cytoplasm. The gene encoding Nop14 spans approximately 25,459 bases and is located on human chromosome 4p16.3, a region that is home to nearly 900 genes, including those associated with various genetic disorders. Understanding Nop14 function is vital, as disruptions in ribosomal RNA processing can lead to a range of diseases, including cancer and ribosomopathies, highlighting the importance of Nop14 in maintaining cellular health and function.

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Nop14 Antibody (B-5) References:

1. Exclusion of the Ellis-van Creveld region on chromosome 4p16 in some families with asphyxiating thoracic dystrophy and short-rib polydactyly syndromes.  |  Krakow, D., et al. 2000. Eur J Hum Genet. 8: 645-8. PMID: 10951528
2. Novel stress-responsive genes EMG1 and NOP14 encode conserved, interacting proteins required for 40S ribosome biogenesis.  |  Liu, PC. and Thiele, DJ. 2001. Mol Biol Cell. 12: 3644-57. PMID: 11694595
3. Phenotypic variations of orpk mutation and chromosomal localization of modifiers influencing kidney phenotype.  |  Sommardahl, C., et al. 2001. Physiol Genomics. 7: 127-34. PMID: 11773599
4. Identification of the gene responsible for the cblA complementation group of vitamin B12-responsive methylmalonic acidemia based on analysis of prokaryotic gene arrangements.  |  Dobson, CM., et al. 2002. Proc Natl Acad Sci U S A. 99: 15554-9. PMID: 12438653
5. NOP14 suppresses breast cancer progression by inhibiting NRIP1/Wnt/β-catenin pathway.  |  Lei, JJ., et al. 2015. Oncotarget. 6: 25701-14. PMID: 26213846
6. LncRNA NOP14-AS1 Promotes Tongue Squamous Cell Carcinoma Progression by Targeting MicroRNA-665/HMGB3 Axis.  |  Li, J., et al. 2021. Cancer Manag Res. 13: 2821-2834. PMID: 33814931
7. The NOP14 nucleolar protein suppresses the function and stemness of melanoma stem-like cells through Wnt/beta-catenin signaling inactivation.  |  Li, J., et al. 2022. Bioengineered. 13: 7648-7658. PMID: 35282769
8. NOP14-mediated ribosome biogenesis is required for mTORC2 activation and predicts rapamycin sensitivity.  |  Yan, X., et al. 2024. J Biol Chem. 300: 105681. PMID: 38272224
9. Huntingtin is ubiquitinated and interacts with a specific ubiquitin-conjugating enzyme.  |  Kalchman, MA., et al. 1996. J Biol Chem. 271: 19385-94. PMID: 8702625
10. Common mutations in the fibroblast growth factor receptor 3 (FGFR 3) gene account for achondroplasia, hypochondroplasia, and thanatophoric dwarfism.  |  Bonaventure, J., et al. 1996. Am J Med Genet. 63: 148-54. PMID: 8723101
11. Autosomal dominant postaxial polydactyly, nail dystrophy, and dental abnormalities map to chromosome 4p16, in the region containing the Ellis-van Creveld syndrome locus.  |  Howard, TD., et al. 1997. Am J Hum Genet. 61: 1405-12. PMID: 9399901
12. Huntingtin protein colocalizes with lesions of neurodegenerative diseases: An investigation in Huntington's, Alzheimer's, and Pick's diseases.  |  Singhrao, SK., et al. 1998. Exp Neurol. 150: 213-22. PMID: 9527890

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Nop14 Antibody (B-5) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Nop14 Antibody (B-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Nop14 Antibody (B-5) Specifications

Alternate Product Name: Nop14

Product Clone Name: B-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 111-138

Epitope Target: NOP14

Epitope Species: human

Product Reactivity/Detection: NOP14

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Nop14 Antibody (B-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin