NOD2 Antibody (2D9): sc-56168

NOD2 Antibody (2D9) is a mouse monoclonal IgG1 kappa light chain antibody that detects the NOD2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. NOD2 (2D9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The NOD2 protein plays a crucial role in the immune response by recognizing bacterial components and activating signaling pathways that lead to the production of pro-inflammatory cytokines. This function is vital for the host′s defense against infections, particularly in the gut, where NOD2 helps maintain intestinal homeostasis and prevent inflammatory diseases such as Crohn′s disease. Structurally, NOD2 consists of two N-terminal caspase recruitment domains, a central nucleotide-binding domain, and multiple C-terminal leucine-rich repeats, which facilitate interaction with other proteins in the signaling cascade. NOD2 expression is predominantly found in monocytes, and NFκB activation in response to bacterial lipopolysaccharides underscores NOD2′s importance in innate immunity.

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NOD2 Antibody (2D9) References:

1. Human CARD4 protein is a novel CED-4/Apaf-1 cell death family member that activates NF-kappaB.  |  Bertin, J., et al. 1999. J Biol Chem. 274: 12955-8. PMID: 10224040
2. Nod1, an Apaf-1-like activator of caspase-9 and nuclear factor-kappaB.  |  Inohara, N., et al. 1999. J Biol Chem. 274: 14560-7. PMID: 10329646
3. An induced proximity model for NF-kappa B activation in the Nod1/RICK and RIP signaling pathways.  |  Inohara, N., et al. 2000. J Biol Chem. 275: 27823-31. PMID: 10880512
4. Molecular cloning and characterization of DEFCAP-L and -S, two isoforms of a novel member of the mammalian Ced-4 family of apoptosis proteins.  |  Hlaing, T., et al. 2001. J Biol Chem. 276: 9230-8. PMID: 11076957
5. Nod2, a Nod1/Apaf-1 family member that is restricted to monocytes and activates NF-kappaB.  |  Ogura, Y., et al. 2001. J Biol Chem. 276: 4812-8. PMID: 11087742
6. NOD1 and NOD2 signalling links ER stress with inflammation.  |  Keestra-Gounder, AM., et al. 2016. Nature. 532: 394-7. PMID: 27007849
7. Palmitoylation of NOD1 and NOD2 is required for bacterial sensing.  |  Lu, Y., et al. 2019. Science. 366: 460-467. PMID: 31649195
8. NOD1 and NOD2 in inflammatory and infectious diseases.  |  Trindade, BC. and Chen, GY. 2020. Immunol Rev. 297: 139-161. PMID: 32677123
9. Palmitoylation restricts SQSTM1/p62-mediated autophagic degradation of NOD2 to modulate inflammation.  |  Zhou, L., et al. 2022. Cell Death Differ. 29: 1541-1551. PMID: 35066577
10. Microbe-mediated intestinal NOD2 stimulation improves linear growth of undernourished infant mice.  |  Schwarzer, M., et al. 2023. Science. 379: 826-833. PMID: 36821686
11. NOD2 attenuates osteoarthritis via reprogramming the activation of synovial macrophages.  |  Li, C., et al. 2023. Arthritis Res Ther. 25: 249. PMID: 38124066
12. Human Nod1 confers responsiveness to bacterial lipopolysaccharides.  |  Inohara, N., et al. 2001. J Biol Chem. 276: 2551-4. PMID: 11058605

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NOD2 Antibody (2D9) has 19 citations in a variety of scientific publications.

Primary antibodies like Anti-NOD2 Antibody (2D9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NOD2 Antibody (2D9) Specifications

Alternate Product Name: NOD2

Product Clone Name: 2D9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 28-301

Epitope Target: Nod2

Epitope Species: human

Product Reactivity/Detection: NOD2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 19 citations in various scientific publications

Available Conjugates/Forms for NOD2 Antibody (2D9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin