NO66 Antibody (3354C5a): sc-81341
- NO66 Antibody (3354C5a) is a mouse monoclonal IgG1, cited in 1 publications, provided at 100 µg/ml
- raised against a recombinant protein corresponding to an internal region of NO66 of human origin
- recommended for detection of NO66 of human origin by WB and IP
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for NO66 Antibody (3354C5a). This work is in progress.
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NO66 Antibody (3354C5a) is a mouse monoclonal IgG1 antibody that detects NO66 in human samples through applications such as western blotting (WB) and immunoprecipitation (IP). NO66, also known as nucleolar protein 66 kDa or C14orf169, is a 641 amino acid protein that plays a crucial role in the synthesis, processing, and assembly of ribosomal RNAs with ribosomal proteins, making NO66 essential for ribosome biogenesis and cellular function. NO66 is primarily localized to nucleoplasmic foci and the nucleolus, specifically within a granular region, which is vital for NO66′s function in the remodeling of heterochromatic regions and the synthesis of the large ribosomal subunit. The presence of one JmjC domain, two putative nuclear localization signals, and several potential phosphorylation sites in NO66 suggests NO66′s involvement in various regulatory processes within the cell. Immunohistochemical studies have shown that NO66 can localize to different subnuclear compartments depending on the cell line, indicating NO66′s dynamic role in cellular contexts. Furthermore, homologs of NO66 have been identified across various species, highlighting NO66′s evolutionary conservation and potential significance in cellular mechanisms. Importantly, NO66 has emerged as a novel therapeutic target oncogene in lung cancer, underscoring NO66′s relevance in cancer research and treatment strategies.
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NO66 Antibody (3354C5a) References:
- NO66, a highly conserved dual location protein in the nucleolus and in a special type of synchronously replicating chromatin. | Eilbracht, J., et al. 2004. Mol Biol Cell. 15: 1816-32. PMID: 14742713
- Polycomb PHF19 binds H3K36me3 and recruits PRC2 and demethylase NO66 to embryonic stem cell genes during differentiation. | Brien, GL., et al. 2012. Nat Struct Mol Biol. 19: 1273-81. PMID: 23160351
- Osterix and NO66 histone demethylase control the chromatin of Osterix target genes during osteoblast differentiation. | Sinha, KM., et al. 2014. J Bone Miner Res. 29: 855-65. PMID: 24115157
- Structure of the JmjC domain-containing protein NO66 complexed with ribosomal protein Rpl8. | Wang, C., et al. 2015. Acta Crystallogr D Biol Crystallogr. 71: 1955-64. PMID: 26327385
- Oncogenic and osteolytic functions of histone demethylase NO66 in castration-resistant prostate cancer. | Sinha, KM., et al. 2019. Oncogene. 38: 5038-5049. PMID: 30858546
- The Histone Demethylase NO66 Induces Glioma Cell Proliferation. | Wang, Q., et al. 2019. Anticancer Res. 39: 6007-6014. PMID: 31704826
- NO66 overexpression rescues ethanol-induced cell apoptosis in human AC16 cardiomyocytes by suppressing PTEN and activating the PI3K/Akt signaling. | Deng, H., et al. 2020. Acta Biochim Biophys Sin (Shanghai). 52: 1093-1101. PMID: 33085743
- The JmjC-domain protein NO66/RIOX-1 affects the balance between proliferation and maturation in acute myeloid leukemia. | Yu, W., et al. 2021. Exp Cell Res. 402: 112566. PMID: 33745927
- First-in-Class Inhibitors of the Ribosomal Oxygenase MINA53. | Nowak, RP., et al. 2021. J Med Chem. 64: 17031-17050. PMID: 34843649
- Substrate selectivity and inhibition of histidine JmjC hydroxylases MINA53 and NO66. | Türkmen, VA., et al. 2023. RSC Chem Biol. 4: 235-243. PMID: 36908702
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NO66 Antibody (3354C5a) | sc-81341 | 100 µg/ml | $339.00 |