NMT1 CRISPR/Cas9 KO Plasmid (m): sc-421916
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NMT1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NMT1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NMT1 Antibody (E-9): sc-393702
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NMT1 CRISPR/Cas9 KO Plasmid (m) | sc-421916 | 20 µg | $397.00 |
Overview
Mouse Nmt1 encodes N-myristoyltransferase 1 (NMT1), a key enzyme that catalyzes co- and post-translational N-myristoylation of proteins bearing an N-terminal glycine. This lipid modification governs membrane association, protein stability, and signal propagation for diverse substrates, thereby influencing processes such as vesicular trafficking, cytoskeletal dynamics, apoptosis, and innate immune signaling. NMT1 activity intersects with pathways controlled by Src-family kinases and small GTPases, shaping cell growth and stress responses. Dysregulated protein myristoylation has been linked to oncogenic signaling states and altered immune and neuronal phenotypes, making Nmt1 a valuable node for mechanistic studies.
NMT1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Nmt1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Nmt1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Nmt1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NMT1 protein expression.
This CRISPR knockout system enables efficient generation of Nmt1-deficient cell models for investigation of NMT1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Nmt1 exon(s) critical for NMT1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Nmt1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NMT1 CRISPR/Cas9 KO Plasmid (m) and NMT1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Nmt1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NMT1 HDR Plasmid (m) and NMT1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Nmt1 homology arms to support homology-directed repair at defined Nmt1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.