NMDA epsilon 1 Antibody (D-8) recommended for detection of NMDAε1 of mouse, rat and human origin by WB, IP, IF and ELISA

NMDAε1 Antibody (D-8): sc-390094

NMDAε1 Antibody (D-8) is rated 5.0 out of 5 by 1.
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Datasheets

    • NMDAε1 Antibody (D-8) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 31-69 within an N-terminal extracellular domain of NMDAε1 of human origin
    • recommended for detection of NMDAε1 of mouse, rat and human origin by WB, IP, IF and ELISA
    • blocking peptide, sc-390094 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.

What application is the blocking peptide sc-390094 P appropriate for?

Asked by: jenni
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24

Are you sure lane A is rat tissue and lane B is mouse tissue? Seems mouse Grin2a should be a little bit bigger than rat Grin2A (see your SC-1468 grin2a antibody C17)

Asked by: roy x
Thank you for your inquiry. These lanes were not side by side in the physical gel, and when the image was cropped and set unfortunately the alignment was off slightly. The bands actually run at visually the same weight, and the image will be reuploaded to reflect this. The predicted weight for both mouse and rat NMDAe1 is 177 kDa, both are glycosylated and we would expect them to migrate at the same weight. We do not have Western Blot data with the goat polyclonal NMDAe1 antibody sc-1468 from a rat tissue sample.
Answered by: Technical Service
Date published: 2016-12-15
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Rated 5 out of 5 by from Good Western blot data of NMDA 1 expression Good Western blot data of NMDA 1 expression in rat brain and mouse brain tissue extracts. -SCBT QC
Date published: 2014-05-18
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