Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NMDAζ1 CRISPR/Cas9 KO Plasmid (h): sc-400593
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NMDAζ1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NMDAζ1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NMDAζ1 Antibody (E-2): sc-518043
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NMDAζ1 CRISPR/Cas9 KO Plasmid (h) | sc-400593 | 20 µg | $397.00 |
Overview
GRIN1 encodes the essential NR1 (NMDAζ1) subunit of N-methyl-D-aspartate (NMDA) receptors, ligand-gated ion channels that mediate Ca²⁺-permeable excitatory neurotransmission. NMDA receptor signaling integrates glutamatergic inputs with membrane depolarization to regulate synaptic plasticity, long-term potentiation, and activity-dependent gene expression programs. Through coupling to postsynaptic density scaffolds and downstream pathways such as CaMK/CREB and MAPK/ERK, GRIN1 influences neuronal development, circuit maturation, and excitotoxic stress responses. Altered GRIN1 function or expression has been implicated in neurodevelopmental and neuropsychiatric phenotypes, seizure susceptibility, and synaptic dysfunction relevant to multiple nervous system disorders.
NMDAζ1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GRIN1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GRIN1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GRIN1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NMDAζ1 protein expression.
This CRISPR knockout system enables efficient generation of GRIN1-deficient cell models for investigation of NMDAζ1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GRIN1 exon(s) critical for NMDAζ1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GRIN1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NMDAζ1 CRISPR/Cas9 KO Plasmid (h) and NMDAζ1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GRIN1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NMDAζ1 HDR Plasmid (h) and NMDAζ1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GRIN1 homology arms to support homology-directed repair at defined GRIN1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.