Nkx-2.2 Antibody (F-2): sc-514161

Nkx-2.2 Antibody (F-2) is a mouse monoclonal IgG1 antibody that detects Nkx-2.2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Nkx-2.2 plays a crucial role in the development and differentiation of various tissues, particularly in the pancreas, where Nkx-2.2 is expressed in alpha, beta, and pancreatic polypeptide cells, but notably absent in delta cells. Nkx-2.2 is essential for the proper differentiation of pancreatic beta cells, which are responsible for insulin production. The significance of Nkx-2.2 is underscored by the severe consequences of homozygous null mutations in mice, leading to hyperglycemia and early postnatal mortality, highlighting Nkx-2.2 as a potential therapeutic target for pancreatic diseases such as diabetes and cancer. anti-Nkx-2.2 antibody (F-2) is an invaluable tool for researchers investigating the molecular mechanisms underlying these conditions, enabling the exploration of Nkx-2.2′s role in cellular signaling pathways and interactions with other proteins involved in pancreatic function and development.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Nkx-2.2 Antibody (F-2) References:

1. Co-localization of Nkx6.2 and Nkx2.2 homeodomain proteins in differentiated myelinating oligodendrocytes.  |  Cai, J., et al. 2010. Glia. 58: 458-68. PMID: 19780200
2. Generation of Nkx2.2:lacZ mice using recombination-mediated cassette exchange technology.  |  Arnes, L., et al. 2012. Genesis. 50: 612-24. PMID: 22539496
3. EWS/FLI utilizes NKX2-2 to repress mesenchymal features of Ewing sarcoma.  |  Fadul, J., et al. 2015. Genes Cancer. 6: 129-43. PMID: 26000096
4. Nkx2.2 is expressed in a subset of enteroendocrine cells with expanded lineage potential.  |  Gross, S., et al. 2015. Am J Physiol Gastrointest Liver Physiol. 309: G975-87. PMID: 26492922
5. Nuclear import of Nkx2-2 is mediated by multiple pathways.  |  Lin, W., et al. 2017. Biochem Biophys Res Commun. 482: 1511-1516. PMID: 27956177
6. NKX2.2, PDX-1 and CDX-2 as potential biomarkers to differentiate well-differentiated neuroendocrine tumors.  |  Yang, MX., et al. 2018. Biomark Res. 6: 15. PMID: 29713473
7. NKL homeobox gene NKX2-2 is aberrantly expressed in Hodgkin lymphoma.  |  Nagel, S., et al. 2018. Oncotarget. 9: 37480-37496. PMID: 30680064
8. Nkx2-2 expressing taste cells in endoderm-derived taste papillae are committed to the type III lineage.  |  Qin, Y., et al. 2021. Dev Biol. 477: 232-240. PMID: 34097879
9. Differential use of the Nkx2.2 NK2 domain in developing pancreatic islets and neurons.  |  Seymour, PA. and Serup, P. 2023. Genes Dev. 37: 451-453. PMID: 37399332
10. The myelination-associated G protein-coupled receptor 37 is regulated by Zfp488, Nkx2.2, and Sox10 during oligodendrocyte differentiation.  |  Schmidt, AL., et al. 2024. Glia. 72: 1304-1318. PMID: 38546197

The preferred Nkx-2.2 antibody is Nkx-2.2 Antibody (D-4): sc-398951.