NKHC1 Antibody (C-11): sc-376452

NKHC1 Antibody (C-11) is a mouse monoclonal IgG2b kappa light chain antibody that detects NKHC1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). NKHC1 (C-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NKHC1, also known as neuronal kinesin heavy chain 1 or KIF5A, plays a crucial role in intracellular transport as a component of a multi-subunit molecular motor complex, facilitating the movement of organelles along microtubules. This function is vital for maintaining neuronal health and function, as proper transport is essential for delivering cellular components necessary for neurotransmission and overall neuronal integrity. Mutations in the gene encoding NKHC1 are linked to autosomal dominant spastic paraplegia 10, highlighting NKHC1′s importance in neurological disorders. NKHC1 exhibits a pan-neuronal distribution within the central nervous system and is particularly enriched in subsets of neurons, with a notable concentration in the perinuclear region of cultured hippocampal neurons. NKHC1′s ability to transport cargo effectively along microtubules underscores this protein′s significance in neuronal function and potential consequences of dysfunction in neurodegenerative diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

NKHC1 Antibody (C-11) References:

1. Defective kinesin heavy chain behavior in mouse kinesin light chain mutants.  |  Rahman, A., et al. 1999. J Cell Biol. 146: 1277-88. PMID: 10491391
2. KIF5C, a novel neuronal kinesin enriched in motor neurons.  |  Kanai, Y., et al. 2000. J Neurosci. 20: 6374-84. PMID: 10964943
3. Abnormal neurofilament transport caused by targeted disruption of neuronal kinesin heavy chain KIF5A.  |  Xia, CH., et al. 2003. J Cell Biol. 161: 55-66. PMID: 12682084
4. Beta-dystrobrevin interacts directly with kinesin heavy chain in brain.  |  Macioce, P., et al. 2003. J Cell Sci. 116: 4847-56. PMID: 14600269
5. Myosin Va and kinesin II motor proteins are concentrated in ribosomal domains (periaxoplasmic ribosomal plaques) of myelinated axons.  |  Sotelo-Silveira, JR., et al. 2004. J Neurobiol. 60: 187-96. PMID: 15266650
6. Evidence of kinesin heavy chain (KIF5A) involvement in pure hereditary spastic paraplegia.  |  Fichera, M., et al. 2004. Neurology. 63: 1108-10. PMID: 15452312
7. GRIF-1 and OIP106, members of a novel gene family of coiled-coil domain proteins: association in vivo and in vitro with kinesin.  |  Brickley, K., et al. 2005. J Biol Chem. 280: 14723-32. PMID: 15644324
8. Molecular basis of dystrobrevin interaction with kinesin heavy chain: structural determinants of their binding.  |  Ceccarini, M., et al. 2005. J Mol Biol. 354: 872-82. PMID: 16288919
9. Mutation in KIF5A can also cause adult-onset hereditary spastic paraplegia.  |  Blair, MA., et al. 2006. Neurogenetics. 7: 47-50. PMID: 16489470
10. Cloning and localization of a conventional kinesin motor expressed exclusively in neurons.  |  Niclas, J., et al. 1994. Neuron. 12: 1059-72. PMID: 7514426

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NKHC1 Antibody (C-11) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-NKHC1 Antibody (C-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NKHC1 Antibody (C-11) Specifications

Alternate Product Name: NKHC1

Product Clone Name: C-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 9-45

Epitope Target: NKHC1

Epitope Species: human

Product Reactivity/Detection: neuronal kinasin heavy chain 1 (NKHC1)

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for NKHC1 Antibody (C-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin