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Nitrotyrosine Antibody (HM.11): sc-32731

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Datasheets
  • Nitrotyrosine Antibody (HM.11) is a mouse monoclonal IgG2b, cited in 35 publications, provided at 100 µg/ml
  • raised against 3-Nitrotyrosine
  • recommended for detection of nitrosylated tyrosine containing proteins by WB, IF and IHC(P)
  • See Nitrotyrosine (39B6): sc-32757 for Nitrotyrosine antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Nitrotyrosine Antibody (HM.11). This work is in progress.

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Nitrotyrosine Antibody (HM.11) is a mouse monoclonal IgG2b light chain antibody that detects nitrotyrosine in non-species reactive samples by western blotting (WB), immunofluorescence (IF), and immunohistochemistry. Anti-nitrotyrosine antibody (HM.11) recognizes a critical biomarker for inflammation and nitric oxide (NO) production, formed when nitric oxide interacts with tyrosine residues in proteins, leading to the formation of peroxynitrite. This post-translational modification indicates oxidative stress and cellular damage, which are pivotal in various pathological conditions. Elevated levels of nitrotyrosine have been associated with numerous inflammatory diseases, including septic shock, rheumatoid arthritis, celiac disease, atherosclerotic plaques, and chronic renal failure, making nitrotyrosine monoclonal antibody (HM.11) an essential tool for researchers investigating the role of nitric oxide in these conditions. Anti-nitrotyrosine antibody (HM.11) enables scientists to gain insights into the mechanisms of NO-dependent damage and the broader implications of nitrotyrosine in disease progression and therapeutic interventions.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Nitrotyrosine Antibody (HM.11)

sc-32731
100 µg/ml
$316.00

What is the total volume provided of Nitrotyrosine (HM.11): sc-32731 monoclonal antibody?

Asked by: Professor Griffin
Thank you for your question. This antibody is provided as 100 µg in 1 ml total volume.
Answered by: Technical Support
Date published: 2017-03-07

Hello, does Nitrotyrosine (HM11) Ab react with Rat tissue? Thank you.

Asked by: NiKa
Thank you for your question. This antibody, Nitrotyrosine (HM11): sc-32731, is not species-specific. The antibody is for specific detection of nitrosylated tyrosine containing proteins, independent of what species the tissue is derived from. We would expect the antibody to react with rat tissue, as long as there is nitrosylated tyrosine containing proteins in the tissue samples being evaluated.
Answered by: Technical Support
Date published: 2016-10-12
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Rated 5 out of 5 by from Cited in well known publicationsWell cited antibody. Published WB and IHCP in mouse, rat, human and rabbit samples
Date published: 2017-02-06
Rated 4 out of 5 by from Produced publishable WB data with human aorticProduced publishable WB data with human aortic endothelial cells and retinal pigment epithelial cells - SCBT Publication Review
Date published: 2015-05-03
Rated 4 out of 5 by from Positive IHCP data gathered with rat kidneyPositive IHCP data gathered with rat kidney -SCBT Publication Review
Date published: 2015-01-19
Rated 4 out of 5 by from Antibody was published for use in IHCP onAntibody was published for use in IHCP on rabbit liver tissue - SCBT Publication Review
Date published: 2013-12-16
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Nitrotyrosine Antibody (HM.11) is rated 4.3 out of 5 by 4.
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