Nitrotyrosine Antibody (HM.11): sc-32731
- Nitrotyrosine Antibody (HM.11) is a mouse monoclonal IgG2b, cited in 35 publications, provided at 100 µg/ml
- raised against 3-Nitrotyrosine
- recommended for detection of nitrosylated tyrosine containing proteins by WB, IF and IHC(P)
- See Nitrotyrosine (39B6): sc-32757 for Nitrotyrosine antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Nitrotyrosine Antibody (HM.11). This work is in progress.
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Nitrotyrosine Antibody (HM.11) is a mouse monoclonal IgG2b light chain antibody that detects nitrotyrosine in non-species reactive samples by western blotting (WB), immunofluorescence (IF), and immunohistochemistry. Anti-nitrotyrosine antibody (HM.11) recognizes a critical biomarker for inflammation and nitric oxide (NO) production, formed when nitric oxide interacts with tyrosine residues in proteins, leading to the formation of peroxynitrite. This post-translational modification indicates oxidative stress and cellular damage, which are pivotal in various pathological conditions. Elevated levels of nitrotyrosine have been associated with numerous inflammatory diseases, including septic shock, rheumatoid arthritis, celiac disease, atherosclerotic plaques, and chronic renal failure, making nitrotyrosine monoclonal antibody (HM.11) an essential tool for researchers investigating the role of nitric oxide in these conditions. Anti-nitrotyrosine antibody (HM.11) enables scientists to gain insights into the mechanisms of NO-dependent damage and the broader implications of nitrotyrosine in disease progression and therapeutic interventions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Nitrotyrosine Antibody (HM.11) References:
- Nitrotyrosine formation and its role in various pathological conditions. | Nakazawa, H., et al. 2000. Free Radic Res. 33: 771-84. PMID: 11237099
- The mechanisms for nitration and nitrotyrosine formation in vitro and in vivo: impact of diet. | Oldreive, C. and Rice-Evans, C. 2001. Free Radic Res. 35: 215-31. PMID: 11697121
- Nitrotyrosine in peripheral vascular disease. | Da Ros, R., et al. 2003. J Thromb Haemost. 1: 382-3. PMID: 12871515
- Detection of specific nitrotyrosine-modified proteins as a marker of oxidative stress in cardiovascular disease. | Viappiani, S. and Schulz, R. 2006. Am J Physiol Heart Circ Physiol. 290: H2167-8. PMID: 16489112
- Assay of 3-nitrotyrosine in tissues and body fluids by liquid chromatography with tandem mass spectrometric detection. | Rabbani, N. and Thornalley, PJ. 2008. Methods Enzymol. 440: 337-59. PMID: 18423229
- Selective Affinity Enrichment of Nitrotyrosine-Containing Peptides for Quantitative Analysis in Complex Samples. | Zhao, Y., et al. 2017. J Proteome Res. 16: 2983-2992. PMID: 28714690
- Blood Levels of Glutamine and Nitrotyrosine in Patients with Chronic Viral Hepatitis. | Murad, H., et al. 2021. Int J Gen Med. 14: 8753-8762. PMID: 34858046
- Selective detection of nitrotyrosine using dual-fluorescent carbon dots. | Camilus, N., et al. 2022. Spectrochim Acta A Mol Biomol Spectrosc. 279: 121444. PMID: 35660143
- Nitrotyrosine as biomarker for reactive nitrogen species. | van der Vliet, A., et al. 1996. Methods Enzymol. 269: 175-84. PMID: 8791647
- Immunohistochemical methods to detect nitrotyrosine. | Viera, L., et al. 1999. Methods Enzymol. 301: 373-81. PMID: 9919586
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nitrotyrosine Antibody (HM.11) | sc-32731 | 100 µg/ml | $322.00 |