Nitrotyrosine Antibody (7A5): sc-81482
- Nitrotyrosine Antibody (7A5) is a mouse monoclonal IgG1, cited in 3 publications, provided at 50 µg/0.5 ml
- raised against 3-Nitrotyrosine
- recommended for detection of nitrosylated tyrosine containing proteins by WB and IP
- See Nitrotyrosine (39B6): sc-32757 for Nitrotyrosine antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Nitrotyrosine Antibody (7A5). This work is in progress.
QUICK LINKS
SEE ALSO...
Nitrotyrosine Antibody (7A5) is a mouse monoclonal IgG1 antibody that detects nitrotyrosine in non-species reactive samples by western blotting (WB) and immunoprecipitation (IP). Anti-nitrotyrosine antibody (7A5) recognizes a critical biomarker for inflammation and nitric oxide (NO) production, formed when nitric oxide interacts with tyrosine residues in proteins, leading to the formation of peroxynitrite. This post-translational modification indicates oxidative stress and cellular damage, which are pivotal in various pathological conditions. Elevated levels of nitrotyrosine have been associated with numerous inflammatory diseases, including septic shock, rheumatoid arthritis, celiac disease, atherosclerotic plaques, and chronic renal failure, making nitrotyrosine a valuable target for research into these conditions. Nitrotyrosine monoclonal antibody (7A5) provides a reliable tool for studying nitrotyrosine in inflammatory processes, helping researchers better understand disease mechanisms and potential therapeutic targets.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Nitrotyrosine Antibody (7A5) References:
- Increased nitrotyrosine in exhaled breath condensate of patients with asthma. | Hanazawa, T., et al. 2000. Am J Respir Crit Care Med. 162: 1273-6. PMID: 11029330
- Nitrotyrosine in human neonatal spinal cord after perinatal asphyxia. | Groenendaal, F., et al. 2008. Neonatology. 93: 1-6. PMID: 17652956
- Mass spectrometric identification of in vivo nitrotyrosine sites in the human pituitary tumor proteome. | Zhan, X. and Desiderio, DM. 2009. Methods Mol Biol. 566: 137-63. PMID: 20058170
- 8-hydroxydeguanosine and nitrotyrosine are prognostic factors in urinary bladder carcinoma. | Soini, Y., et al. 2011. Int J Clin Exp Pathol. 4: 267-75. PMID: 21487522
- Characterization of nitrotyrosine as a biomarker for arthritis and joint injury. | Misko, TP., et al. 2013. Osteoarthritis Cartilage. 21: 151-6. PMID: 23025928
- Direct detection of nitrotyrosine-containing proteins using an aniline-based oxidative coupling strategy. | Sangsuwan, R., et al. 2016. Chem Commun (Camb). 52: 10036-9. PMID: 27447346
- pH-Dependent Nitrotyrosine Formation in Ribonuclease A is Enhanced in the Presence of Polyethylene Glycol (PEG). | Roy, P., et al. 2019. Chem Asian J. 14: 4780-4792. PMID: 31591811
- Identification of Human IDO1 Enzyme Activity by Using Genetically Encoded Nitrotyrosine. | Zheng, Z., et al. 2020. Chembiochem. 21: 1593-1596. PMID: 31944493
- Stacking based ensemble learning framework for identification of nitrotyrosine sites. | Parvez, A., et al. 2024. Comput Biol Med. 183: 109200. PMID: 39366143
- Lipopolysaccharide-induced increase in plasma nitrotyrosine concentrations in rats. | Kamisaki, Y., et al. 1997. Biochim Biophys Acta. 1362: 24-8. PMID: 9434096
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nitrotyrosine Antibody (7A5) | sc-81482 | 50 µg/0.5 ml | $322.00 |