Nitrotyrosine Antibody (2A12): sc-65384
- Nitrotyrosine Antibody (2A12) is a mouse monoclonal IgG1, cited in 1 publications, provided at 100 µg/ml
- raised against 3-Nitrotyrosine
- recommended for detection of nitrosylated tyrosine containing proteins by WB and IP
- See Nitrotyrosine (39B6): sc-32757 for Nitrotyrosine antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Nitrotyrosine Antibody (2A12). This work is in progress.
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Nitrotyrosine Antibody (2A12) is a mouse monoclonal IgG1 antibody that detects nitrotyrosine in non-species reactive samples by western blotting (WB) and immunoprecipitation (IP). Nitrotyrosine is a critical biomarker for inflammation and nitric oxide (NO) production, formed when NO interacts with tyrosine residues in proteins, leading to the formation of peroxynitrite. This post-translational modification can indicate oxidative stress and cellular damage, which are pivotal in various pathological conditions. Elevated levels of nitrotyrosine have been associated with numerous inflammatory diseases, including septic shock, rheumatoid arthritis, celiac disease, atherosclerotic plaques, and chronic renal failure, making anti-nitrotyrosine antibody (2A12) an essential tool for researchers investigating the role of nitric oxide in these conditions. By using nitrotyrosine monoclonal antibody (2A12), scientists can gain insights into the mechanisms of NO-dependent damage and the broader implications of nitrotyrosine in disease progression and therapeutic interventions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Nitrotyrosine Antibody (2A12) References:
- A review of approaches to the analysis of 3-nitrotyrosine. | Duncan, MW. 2003. Amino Acids. 25: 351-61. PMID: 14661096
- Nitrotyrosine in brain tissue of neonates after perinatal asphyxia. | Groenendaal, F., et al. 2006. Arch Dis Child Fetal Neonatal Ed. 91: F429-33. PMID: 16835259
- Identification of 3-nitrotyrosine-modified brain proteins by redox proteomics. | Butterfield, DA. and Sultana, R. 2008. Methods Enzymol. 440: 295-308. PMID: 18423226
- Quantification of nitrotyrosine in nitrated proteins. | Yang, H., et al. 2010. Anal Bioanal Chem. 397: 879-86. PMID: 20300739
- Decrease in 3-nitrotyrosine in the amniotic fluid of women with cytomegalovirus infection. | Gaj, Z., et al. 2014. J Obstet Gynaecol. 34: 251-4. PMID: 24483707
- 3-Nitrotyrosine as a biomarker for vascular involvement in Fabry disease. | Joly, DA. and Grünfeld, JP. 2014. Kidney Int. 86: 5-7. PMID: 24978372
- pNitro-Tyr-PseAAC: Predict Nitrotyrosine Sites in Proteins by Incorporating Five Features into Chou's General PseAAC. | Ghauri, AW., et al. 2018. Curr Pharm Des. 24: 4034-4043. PMID: 30479209
- Decreased proteasomal cleavage at nitrotyrosine sites in proteins and peptides. | Ott, C., et al. 2021. Redox Biol. 46: 102106. PMID: 34455147
- Revisiting the role of 3-nitrotyrosine residues in the formation of alpha-synuclein oligomers and fibrils. | Chavarría, C., et al. 2024. Arch Biochem Biophys. 752: 109858. PMID: 38104957
- Nitrotyrosine in plasma of celiac disease patients as detected by a new sandwich ELISA. | ter Steege, JC., et al. 1998. Free Radic Biol Med. 25: 953-63. PMID: 9840741
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nitrotyrosine Antibody (2A12) | sc-65384 | 100 µg/ml | $322.00 |