nitrated Tau Antibody (Tau-nY29): sc-66177
- nitrated Tau Antibody (Tau-nY29) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 25-37 surrounding the nitrated Tyr29 residue of nitrated Tau of human origin
- recommended for detection of Tau nitrated at Tyr29 of human origin by WB, IP and IF; non cross-reactive with wild-type Tau, Tau mutants singly nitrated at Tyr18, Tyr197, and Tyr394, or other proteins known to be nitrated in neurodegenerative diseases
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for nitrated Tau Antibody (Tau-nY29) for WB applications. This reagent is now offered in a bundle with nitrated Tau Antibody (Tau-nY29) (see ordering information below).
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nitrated Tau Antibody (Tau-nY29) is a mouse monoclonal IgG1 kappa light chain antibody developed to specifically recognize Tau protein nitrated at the tyrosine 29 residue. anti-Tau antibody (Tau-nY29) targets an epitope encompassing amino acids 25-37 surrounding the nitrated Tyr29 of human Tau, ensuring precise detection of this modified form. Tau, also known as MAPT (microtubule-associated protein tau), or TAU, is a 758 amino acid protein primarily localized in the neuronal cytoplasm, cytoskeleton, and cell membrane. Strategic location is crucial for stabilizing microtubules, which maintain the structural integrity of neurons and facilitate essential intracellular transport processes. Nitration of Tau at Tyr29 can disrupt microtubule-binding ability, potentially leading to impaired neuronal function and contributing to the pathology of neurodegenerative diseases. Tau monoclonal antibody (Tau-nY29) is reactive with Tau nitrated at Tyr29 in human samples and is effective in applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Importantly, anti-Tau antibody (Tau-nY29) does not cross-react with wild-type Tau, Tau mutants nitrated at other tyrosine residues, or other nitrated proteins associated with neurodegenerative conditions. By enabling researchers to investigate the role of Tau nitration, Tau monoclonal antibody (Tau-nY29) supports the study of molecular mechanisms underlying diseases like Alzheimer′s, providing valuable insights into neuronal dysfunction and degeneration.
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nitrated Tau Antibody (Tau-nY29) References:
- Nitration of tau protein is linked to neurodegeneration in tauopathies. | Horiguchi, T., et al. 2003. Am J Pathol. 163: 1021-31. PMID: 12937143
- The nitration of tau protein in neurone-like PC12 cells. | Cappelletti, G., et al. 2004. FEBS Lett. 562: 35-9. PMID: 15043998
- Nitration and oligomerization of tau induced by peroxynitrite inhibit its microtubule-binding activity. | Zhang, YJ., et al. 2005. FEBS Lett. 579: 2421-7. PMID: 15848182
- Tau nitration occurs at tyrosine 29 in the fibrillar lesions of Alzheimer's disease and other tauopathies. | Reynolds, MR., et al. 2006. J Neurosci. 26: 10636-45. PMID: 17050703
- A possible link between astrocyte activation and tau nitration in Alzheimer's disease. | Reyes, JF., et al. 2008. Neurobiol Dis. 31: 198-208. PMID: 18562203
- MAPT/Tau accumulation represses autophagy flux by disrupting IST1-regulated ESCRT-III complex formation: a vicious cycle in Alzheimer neurodegeneration. | Feng, Q., et al. 2020. Autophagy. 16: 641-658. PMID: 31223056
- Microtubule-associated protein tau in murine kidney: role in podocyte architecture. | Vallés-Saiz, L., et al. 2022. Cell Mol Life Sci. 79: 97. PMID: 35084555
- Phase separation of the microtubule-associated protein tau. | Chakraborty, P. and Zweckstetter, M. 2022. Essays Biochem. 66: 1013-1021. PMID: 36251053
- Endolysosomal impairment by binding of amyloid beta or MAPT/Tau to V-ATPase and rescue via the HYAL-CD44 axis in Alzheimer disease. | Kim, SH., et al. 2023. Autophagy. 19: 2318-2337. PMID: 36843263
- Microtubule-associated protein tau, paired helical filaments, and phosphorylation. | Mandelkow, EM., et al. 1993. Ann N Y Acad Sci. 695: 209-16. PMID: 7694533
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
nitrated Tau Antibody (Tau-nY29) | sc-66177 | 200 µg/ml | $316.00 | |||
nitrated Tau Antibody (Tau-nY29): m-IgG Fc BP-HRP Bundle | sc-551948 | 200 µg Ab; 10 µg BP | $354.00 |