NIRF Antibody (C-10): sc-398953

NIRF Antibody (C-10) is a mouse monoclonal IgG1 antibody that detects NIRF protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NIRF (C-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NIRF protein, also known as Np95/ICBP90-like RING finger protein or E3 ubiquitin-protein ligase UHRF2, plays a crucial role in cell cycle regulation, particularly in the G1/S transition. Located primarily in the nucleus, NIRF contains a PHD finger, two RING fingers, a ubiquitin-like domain, and a YDG/SRA domain, which are essential for functioning as a negative regulator of cell proliferation. Unlike structural homolog UHRF1, NIRF associates with the Cdk2-cyclin complex in dephosphorylated form, inducing G1 arrest and preventing cells from entering S-phase. This regulatory mechanism is vital for maintaining proper cell cycle progression and preventing uncontrolled cell division, which can lead to tumorigenesis. Additionally, NIRF can ubiquitinate specific proteins such as PCNP and recruit histone deacetylase 1 (HDAC1) through SRA domain, further emphasizing importance in cellular regulation. NIRF overexpression has been shown to increase the population of cells in G1 phase, highlighting significant role in cell cycle control.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

NIRF Antibody (C-10) References:

1. NIRF, a novel RING finger protein, is involved in cell-cycle regulation.  |  Mori, T., et al. 2002. Biochem Biophys Res Commun. 296: 530-6. PMID: 12176013
2. NIRF is a ubiquitin ligase that is capable of ubiquitinating PCNP, a PEST-containing nuclear protein.  |  Mori, T., et al. 2004. FEBS Lett. 557: 209-14. PMID: 14741369
3. NIRF induces G1 arrest and associates with Cdk2.  |  Li, Y., et al. 2004. Biochem Biophys Res Commun. 319: 464-8. PMID: 15178429
4. ICBP90, an E2F-1 target, recruits HDAC1 and binds to methyl-CpG through its SRA domain.  |  Unoki, M., et al. 2004. Oncogene. 23: 7601-10. PMID: 15361834
5. TCR pathway involves ICBP90 gene down-regulation via E2F binding sites.  |  Abbady, AQ., et al. 2005. Biochem Pharmacol. 70: 570-9. PMID: 15964557
6. Isolation and Characterization of a Novel Human Radiosusceptibility Gene, NP95.  |  Muto, M., et al. 2006. Radiat Res. 166: 723-33. PMID: 17067204
7. VIM1, a methylcytosine-binding protein required for centromeric heterochromatinization.  |  Woo, HR., et al. 2007. Genes Dev. 21: 267-77. PMID: 17242155
8. The UHRF family: oncogenes that are drugable targets for cancer therapy in the near future?  |  Bronner, C., et al. 2007. Pharmacol Ther. 115: 419-34. PMID: 17658611
9. Site-specifically labeled CA19.9-targeted immunoconjugates for the PET, NIRF, and multimodal PET/NIRF imaging of pancreatic cancer.  |  Houghton, JL., et al. 2015. Proc Natl Acad Sci U S A. 112: 15850-5. PMID: 26668398
10. NIRF Heptamethine Cyanine Dye Nanocomplexes for Multi Modal Theranosis of Tumors.  |  Thomas, RG. and Jeong, YY. 2017. Chonnam Med J. 53: 83-94. PMID: 28584786
11. Targeted NIRF/MR dual-mode imaging of breast cancer brain metastasis using BRBP1-functionalized ultra-small iron oxide nanoparticles.  |  Du, J., et al. 2020. Mater Sci Eng C Mater Biol Appl. 116: 111188. PMID: 32806329
12. Albumin binding improves nanobody pharmacokinetics for dual-modality PET/NIRF imaging of CEACAM5 in colorectal cancer models.  |  Song, W., et al. 2023. Eur J Nucl Med Mol Imaging. 50: 2591-2594. PMID: 37191678

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NIRF Antibody (C-10) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-NIRF Antibody (C-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NIRF Antibody (C-10) Specifications

Alternate Product Name: NIRF

Product Clone Name: C-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: 144-208

Epitope Target: NIRF

Epitope Species: human

Product Reactivity/Detection: NIRF

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for NIRF Antibody (C-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin