NIPSNAP1 Antibody (H-9): sc-515197
- NIPSNAP1 Antibody (H-9) is a mouse monoclonal IgG1 κ NIPSNAP1 antibody, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 95-113 within an internal region of NIPSNAP1 of human origin
- NIPSNAP1 Antibody (H-9) is recommended for detection of NIPSNAP1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-NIPSNAP1 Antibody (H-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for NIPSNAP1 Antibody (H-9) for WB applications. These reagents are now offered in bundles with NIPSNAP1 Antibody (H-9) (see ordering information below).
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NIPSNAP1 Antibody (H-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects NIPSNAP1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NIPSNAP1 (H-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NIPSNAP1, or 4-nitrophenylphosphatase domain and non-neuronal SNAP25-like 1, is a 228 amino acid protein that plays a crucial role in modulating the activity of transient receptor potential (TRP) channels, particularly TRPV6, which is essential for calcium homeostasis in various tissues such as the pancreas and placenta. The inhibition of TRPV6 currents by NIPSNAP1 is significant because NIPSNAP1 regulates calcium entry across the plasma membrane, a process vital for numerous cellular functions, including muscle contraction, neurotransmitter release, and hormone secretion. NIPSNAP1 does not alter the membrane expression of TRPV6, indicating that NIPSNAP1′s inhibitory effect is mediated through a mechanism that does not involve reducing the number of channels on the cell surface. This unique regulatory function of NIPSNAP1 highlights NIPSNAP1′s importance in maintaining calcium balance and suggests potential implications in various physiological and pathological processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
NIPSNAP1 Antibody (H-9) References:
- Identification of a NIPSNAP homologue as host cell target for Salmonella virulence protein SpiC. | Lee, AH., et al. 2002. Cell Microbiol. 4: 739-50. PMID: 12427096
- Expression pattern and raft association of NIPSNAP3 and NIPSNAP4, highly homologous proteins encoded by genes in close proximity to the ATP-binding cassette transporter A1. | Buechler, C., et al. 2004. Genomics. 83: 1116-24. PMID: 15177564
- Expression of calpastatin, minopontin, NIPSNAP1, rabaptin-5 and neuronatin in the phenylketonuria (PKU) mouse brain: possible role on cognitive defect seen in PKU. | Surendran, S., et al. 2005. Neurochem Int. 46: 595-9. PMID: 15863237
- Identification of Nipsnap1 as a novel auxiliary protein inhibiting TRPV6 activity. | Schoeber, JP., et al. 2008. Pflugers Arch. 457: 91-101. PMID: 18392847
- [Identification of proteins in complexes with alpha-latrotoxin receptors]. | Serova, OV., et al. 2008. Bioorg Khim. 34: 747-53. PMID: 19088747
- Lysine acetylation targets protein complexes and co-regulates major cellular functions. | Choudhary, C., et al. 2009. Science. 325: 834-40. PMID: 19608861
- Sequence variants in four candidate genes (NIPSNAP1, GBAS, CHCHD1 and METT11D1) in patients with combined oxidative phosphorylation system deficiencies. | Smits, P., et al. 2010. J Inherit Metab Dis. 33 Suppl 3: S13-9. PMID: 24137763
- Characterization of the human NIPSNAP1 gene from 22q12: a member of a novel gene family. | Seroussi, E., et al. 1998. Gene. 212: 13-20. PMID: 9661659
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NIPSNAP1 Antibody (H-9) | sc-515197 | 200 µg/ml | $322.00 | |||
NIPSNAP1 Antibody (H-9): m-IgG Fc BP-HRP Bundle | sc-531309 | 200 µg Ab; 10 µg BP | $361.00 | |||
NIPSNAP1 Antibody (H-9): m-IgGκ BP-HRP Bundle | sc-524967 | 200 µg Ab, 40 µg BP | $361.00 | |||
NIPSNAP1 Antibody (H-9): m-IgG1 BP-HRP Bundle | sc-544478 | 200 µg Ab; 20 µg BP | $361.00 | |||
NIPSNAP1 Antibody (H-9) AC | sc-515197 AC | 500 µg/ml, 25% agarose | $424.00 | |||
NIPSNAP1 Antibody (H-9) HRP | sc-515197 HRP | 200 µg/ml | $322.00 | |||
NIPSNAP1 Antibody (H-9) FITC | sc-515197 FITC | 200 µg/ml | $336.00 | |||
NIPSNAP1 Antibody (H-9) PE | sc-515197 PE | 200 µg/ml | $349.00 | |||
NIPSNAP1 Antibody (H-9) Alexa Fluor® 488 | sc-515197 AF488 | 200 µg/ml | $364.00 | |||
NIPSNAP1 Antibody (H-9) Alexa Fluor® 546 | sc-515197 AF546 | 200 µg/ml | $364.00 | |||
NIPSNAP1 Antibody (H-9) Alexa Fluor® 594 | sc-515197 AF594 | 200 µg/ml | $364.00 | |||
NIPSNAP1 Antibody (H-9) Alexa Fluor® 647 | sc-515197 AF647 | 200 µg/ml | $364.00 | |||
NIPSNAP1 Antibody (H-9) Alexa Fluor® 680 | sc-515197 AF680 | 200 µg/ml | $364.00 | |||
NIPSNAP1 Antibody (H-9) Alexa Fluor® 790 | sc-515197 AF790 | 200 µg/ml | $364.00 | |||
NIPSNAP1 (H-9) Neutralizing Peptide | sc-515197 P | 100 µg/0.5 ml | $69.00 |