NIPP1 Antibody (A-11): sc-393991

NIPP1 Antibody (A-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects NIPP1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-NIPP1 antibody (A-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NIPP1, known as nuclear inhibitor of protein phosphatase 1, plays a crucial role in regulating cellular processes such as pre-mRNA splicing and cell proliferation, making NIPP1 essential for proper gene expression and cellular function. NIPP1 contains a forkhead-associated (FHA) domain, which is significant for interactions with protein kinases and transcription factors, thereby influencing various signaling pathways. NIPP1 gene encodes three isoforms—α, β, and γ—resulting from alternative splicing, each exhibiting distinct RNA binding activities and phosphatase inhibition capabilities. Notably, γ isoform functions as a magnesium-dependent endoribonuclease, primarily found in B cells and T lymphocytes, and is responsible for cleaving RNA strands, which is vital for RNA processing and regulation. α and β isoforms are predominantly localized in the brain and kidney, highlighting diverse functional roles of NIPP1 across different tissues. Inactivation of NIPP1 occurs through phosphorylation at Serine 199 or Serine 204, which can modulate NIPP1 activity and interactions with other proteins. NIPP1 interacts with several key proteins, including CDc5L, SAP 155, MELK, and EED, further underscoring NIPP1′s importance in cellular signaling and regulatory networks.

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NIPP1 Antibody (A-11) References:

1. Organization and alternate splice products of the gene encoding nuclear inhibitor of protein phosphatase-1 (NIPP-1).  |  Van Eynde, A., et al. 1999. Eur J Biochem. 261: 291-300. PMID: 10103062
2. Phosphorylation-dependent interaction between the splicing factors SAP155 and NIPP1.  |  Boudrez, A., et al. 2002. J Biol Chem. 277: 31834-41. PMID: 12105215
3. Functional interaction between nuclear inhibitor of protein phosphatase type 1 (NIPP1) and protein phosphatase type 1 (PP1) in Drosophila: consequences of over-expression of NIPP1 in flies and suppression by co-expression of PP1.  |  Parker, L., et al. 2002. Biochem J. 368: 789-97. PMID: 12358598
4. Inhibition of spliceosome assembly by the cell cycle-regulated protein kinase MELK and involvement of splicing factor NIPP1.  |  Vulsteke, V., et al. 2004. J Biol Chem. 279: 8642-7. PMID: 14699119
5. The nuclear scaffold protein NIPP1 is essential for early embryonic development and cell proliferation.  |  Van Eynde, A., et al. 2004. Mol Cell Biol. 24: 5863-74. PMID: 15199142
6. Dephosphorylation of CDK9 by protein phosphatase 2A and protein phosphatase-1 in Tat-activated HIV-1 transcription.  |  Ammosova, T., et al. 2005. Retrovirology. 2: 47. PMID: 16048649
7. Regulation of protein phosphatase 1gamma activity in hypoxia through increased interaction with NIPP1: implications for cellular metabolism.  |  Comerford, KM., et al. 2006. J Cell Physiol. 209: 211-8. PMID: 16826568
8. The transcriptional repression by NIPP1 is mediated by Polycomb group proteins.  |  Roy, N., et al. 2007. Biochim Biophys Acta. 1769: 541-5. PMID: 17804093
9. Molecular cloning of NIPP-1, a nuclear inhibitor of protein phosphatase-1, reveals homology with polypeptides involved in RNA processing.  |  Van Eynde, A., et al. 1995. J Biol Chem. 270: 28068-74. PMID: 7499293

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NIPP1 Antibody (A-11) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-NIPP1 Antibody (A-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NIPP1 Antibody (A-11) Specifications

Alternate Product Name: NIPP1

Product Clone Name: A-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 221-351

Epitope Target: NIPP1

Epitope Species: human

Product Reactivity/Detection: NIPP1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for NIPP1 Antibody (A-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin